蜀柏毒蛾核型多角体病毒结构多肽及基因组酶切分析
作者：赵小东 周建华 丁清泉
单位：赵小东 丁清泉(中国科学院武汉病毒研究所，武汉 430071)；周建华(四川省林业科学院，成都 610081)
关键词：蜀柏毒蛾核型多角体病毒；结构多肽；内切酶图谱
中国病毒学000308摘要：对蜀柏毒蛾核型多角体病毒(Parocneria orienta Nuclear polyhedrovirus,简称PaorNPV)形态结构、结构多肽、限制性内切酶图谱等特性进行了研究。采用不连续系统垂直板SDS-PAGE分析了PaorNPV的多角体蛋白、病毒粒子结构多肽。应用5种限制性内切酶对PaorNPV基因组DNA进行了酶切分析。结果表明：经热处理的多角体蛋白仅有一条带，分子量为31.5 kD，不经热处理的多角体蛋白有三条带，分子量分别为31.5 kD、29.1 kD、28.6 kD；病毒粒子包含有25种结构多肽，分子量范围在17.6-114.6 kD之间。PaorNPV DNA经BamH I。EcoR I、HindⅢ、Pst I和Xho I酶切分别产生9、12、12、12和14条片段。基因组大小平均为124.6 kb。
, 百拇医药
中图分类号：S763.42 文献标识码：A
文章编号：1003-5125(2000)03-0249-06
Analysis of Structural Polypeptides and Restriction Patterns
of Parocneria orienta Nuclear polyhedrovirus
ZHAO Xiao-dong，DING Qing-quan
(Wuhan Institute of Virology, Academia Sinica, Wuhan 430071)
ZHOU Jian-hua
, 百拇医药
(Sichuan Academy of Forest, Chendu 610081)
Abstract: The shape, structure, structural polypeptides and restriction pattern of Parocneria orienta Nuclear polyhedrovirus (PaorNPV)was studied. Assayed by vertical slab SDS-PAGE, polyhedrin treated by heat contained a single polypeptide with molecular weight of 31.5 kD, while polyhedrin untreated by heat contained three polypeptides with molecular weight of 31.5 kD, 29.1 kD, 28.6 kD respectively; virion contained 25 structural polypeptides with molecular weight range from 17.6 kD to 114.6 kD. Digestion of PaorNPV DNA with restriction endonucleases, BamH I, EcoR I, Hind Ⅲ, Pst I and Xho I, resulted in 9,12,12,12 and 14 fragments, respectively. The PaorNPV genome was about 124.6 kilobasepairs (kb). Ⅲ, Pst I and Xho I, resulted in 9,12,12,12 and 14 fragments, respectively. The PaorNPV genome was about 124.6 kilobasepairs (kb).
, http://www.100md.com
Key words: Parocneria orienta Nuclear polyhedrovirus (PaorNPV), Structural polypeptides, Restriction endonucleas pattern
第一作者简介：赵小东(1972年-)，男，湖北黄冈人，在读研究生，研究方向：分子病毒学。
参考文献
[1] 何礼.蜀柏毒蛾生物学特性及防治[J].昆虫知识，1998，35(2)：90-93
[2] 陈新文，彭辉银，王根，等.蜀柏毒蛾核型多角体病毒的分离鉴定[J].中国病毒学，1991，6(4)：379-381
[3] 周建华，唐孟佳，秦严昌，等.蜀柏毒蛾核型多角体病毒杀虫剂防治效果研究[J].四川林业科技，1992，13(4)：31-32
, 百拇医药
[4] 王根，张传溪，金伟，等.棉铃虫核型多角体病毒基因组限制酶酶切分析及其多角体基因的定位[J].应用与环境生物学报.1996，2(4)：387-392
[5] 刘年翠，孟小林.油桐尺蠖核型多角体病毒结构多肽的分析[J].病毒学杂志，1988，1：48-52
[6] 孙修炼，张光裕.棉铃虫核型多角体病毒四个分离株的比较研究[J].中国病毒学，1994，9(4)：309-318
[7] Leammli UK. Cleavage of structural protein during the assembly of the head of bacteriophage 7[J]. Nature 1970,227:680-685
[8] 李枚.中国棉铃虫单粒包埋核型多角体病毒基因组结构及同源重复区分析[D].武汉：中国科学院武汉病毒所，1998
, 百拇医药
[9] Sambrook J, Rristch EF, Maniatis T. Molecular Cloning, 2nd, CHS Press,1989
[10] Eppstein DA, Thoma TA, Scott HA. Degradation of matrix protein from a nuclear polyhedrosis virus of Tichoplusia ni by an endogenous protease [J]. Virology, 1975,67:591-594
[11] Eppstein DA, Thoma JA. Alkaline protease associated with matrix protein of a virus infecting the cabbage looper[J]. Biochem Biophy Res commun, 1975,62:478-584
, http://www.100md.com
[12] Keith AH, Payne CC. The properties of three baculoviruses from closely related hosts [J]. Virology, 1977,79:14-31
[13] Payne CC, Kacmakoff J. Alkaline protease associated with virus partials of nuclear polyhedrosis virus: assay, purification and propeties [J]. J Virol, 1978,26:84-92
[14] Summer MD, Smith GE. Baculovirus, structural polypeptides [J]. virology,1978,84:390-402
[15] Bell CD, Orlob GB. Serological studies on virions and polyhedron protein of a nuclear polyhedrosis virus of the cabbage looper, Trichoplusia ni [J]. Virology,1977,78:162-172
收稿日期：1999-07-23，修回日期：2000-02-24, 百拇医药
单位：赵小东 丁清泉(中国科学院武汉病毒研究所，武汉 430071)；周建华(四川省林业科学院，成都 610081)
关键词：蜀柏毒蛾核型多角体病毒；结构多肽；内切酶图谱
中国病毒学000308摘要：对蜀柏毒蛾核型多角体病毒(Parocneria orienta Nuclear polyhedrovirus,简称PaorNPV)形态结构、结构多肽、限制性内切酶图谱等特性进行了研究。采用不连续系统垂直板SDS-PAGE分析了PaorNPV的多角体蛋白、病毒粒子结构多肽。应用5种限制性内切酶对PaorNPV基因组DNA进行了酶切分析。结果表明：经热处理的多角体蛋白仅有一条带，分子量为31.5 kD，不经热处理的多角体蛋白有三条带，分子量分别为31.5 kD、29.1 kD、28.6 kD；病毒粒子包含有25种结构多肽，分子量范围在17.6-114.6 kD之间。PaorNPV DNA经BamH I。EcoR I、HindⅢ、Pst I和Xho I酶切分别产生9、12、12、12和14条片段。基因组大小平均为124.6 kb。
, 百拇医药
中图分类号：S763.42 文献标识码：A
文章编号：1003-5125(2000)03-0249-06
Analysis of Structural Polypeptides and Restriction Patterns
of Parocneria orienta Nuclear polyhedrovirus
ZHAO Xiao-dong，DING Qing-quan
(Wuhan Institute of Virology, Academia Sinica, Wuhan 430071)
ZHOU Jian-hua
, 百拇医药
(Sichuan Academy of Forest, Chendu 610081)
Abstract: The shape, structure, structural polypeptides and restriction pattern of Parocneria orienta Nuclear polyhedrovirus (PaorNPV)was studied. Assayed by vertical slab SDS-PAGE, polyhedrin treated by heat contained a single polypeptide with molecular weight of 31.5 kD, while polyhedrin untreated by heat contained three polypeptides with molecular weight of 31.5 kD, 29.1 kD, 28.6 kD respectively; virion contained 25 structural polypeptides with molecular weight range from 17.6 kD to 114.6 kD. Digestion of PaorNPV DNA with restriction endonucleases, BamH I, EcoR I, Hind Ⅲ, Pst I and Xho I, resulted in 9,12,12,12 and 14 fragments, respectively. The PaorNPV genome was about 124.6 kilobasepairs (kb). Ⅲ, Pst I and Xho I, resulted in 9,12,12,12 and 14 fragments, respectively. The PaorNPV genome was about 124.6 kilobasepairs (kb).
, http://www.100md.com
Key words: Parocneria orienta Nuclear polyhedrovirus (PaorNPV), Structural polypeptides, Restriction endonucleas pattern
第一作者简介：赵小东(1972年-)，男，湖北黄冈人，在读研究生，研究方向：分子病毒学。
参考文献
[1] 何礼.蜀柏毒蛾生物学特性及防治[J].昆虫知识，1998，35(2)：90-93
[2] 陈新文，彭辉银，王根，等.蜀柏毒蛾核型多角体病毒的分离鉴定[J].中国病毒学，1991，6(4)：379-381
[3] 周建华，唐孟佳，秦严昌，等.蜀柏毒蛾核型多角体病毒杀虫剂防治效果研究[J].四川林业科技，1992，13(4)：31-32
, 百拇医药
[4] 王根，张传溪，金伟，等.棉铃虫核型多角体病毒基因组限制酶酶切分析及其多角体基因的定位[J].应用与环境生物学报.1996，2(4)：387-392
[5] 刘年翠，孟小林.油桐尺蠖核型多角体病毒结构多肽的分析[J].病毒学杂志，1988，1：48-52
[6] 孙修炼，张光裕.棉铃虫核型多角体病毒四个分离株的比较研究[J].中国病毒学，1994，9(4)：309-318
[7] Leammli UK. Cleavage of structural protein during the assembly of the head of bacteriophage 7[J]. Nature 1970,227:680-685
[8] 李枚.中国棉铃虫单粒包埋核型多角体病毒基因组结构及同源重复区分析[D].武汉：中国科学院武汉病毒所，1998
, 百拇医药
[9] Sambrook J, Rristch EF, Maniatis T. Molecular Cloning, 2nd, CHS Press,1989
[10] Eppstein DA, Thoma TA, Scott HA. Degradation of matrix protein from a nuclear polyhedrosis virus of Tichoplusia ni by an endogenous protease [J]. Virology, 1975,67:591-594
[11] Eppstein DA, Thoma JA. Alkaline protease associated with matrix protein of a virus infecting the cabbage looper[J]. Biochem Biophy Res commun, 1975,62:478-584
, http://www.100md.com
[12] Keith AH, Payne CC. The properties of three baculoviruses from closely related hosts [J]. Virology, 1977,79:14-31
[13] Payne CC, Kacmakoff J. Alkaline protease associated with virus partials of nuclear polyhedrosis virus: assay, purification and propeties [J]. J Virol, 1978,26:84-92
[14] Summer MD, Smith GE. Baculovirus, structural polypeptides [J]. virology,1978,84:390-402
[15] Bell CD, Orlob GB. Serological studies on virions and polyhedron protein of a nuclear polyhedrosis virus of the cabbage looper, Trichoplusia ni [J]. Virology,1977,78:162-172
收稿日期：1999-07-23，修回日期：2000-02-24, 百拇医药