肾综合征出血热病毒对肾小管上皮细胞损伤的研究
作者：姚丽君 邓安国 黄蓓 李元莉
单位：430022武汉，同济医科大学附属协和医院肾内科(E-mail：yaoli@Public.wh.hb.cn)
关键词：出血热；肾综合征；肾小管上皮细胞；出血热病毒
中华肾脏病杂志000309摘 要：目的研究肾综合征出血热病毒(HFRSV)对肾小管上皮细胞(RTC)的致病作用。方法应用HFRSV感染体外培养的人胚RTC(HFRTC)，采用原位分子杂交技术检测HFRTC内的HFRSV并作台盼蓝染色、透射电镜(TEM)观察。结果(1)HPRSV能够直接感染培养的HFRTC；(2)从第7天起被感染的HPRTC死亡率明显高于对照组；(3)TEM观察表明HFRSV可致HPRTC的细胞膜、细胞器损害。结论HFRSV能够直接损害体外培养的HPRTC，其对RTC的损害作用可能直接参予肾综出血热(HPRS)的发病。
, 百拇医药
The study of cytopathogenic effect of hemorrhagic fever with renal syndrome virus on renal tubular cells
YAO Lijun,DENG Anguo,HUANG Pei
(Department of Nephrology, Union Hospital , Tongji Medical University, Wuhan 430022, China)
Abstract：Objective To study the cytopathogcnic effect of epidemic hemorrhagic fever with renal syndrome virus (HFRSV) on renal tubular cells(RTC) . Methods Human fetal renal tubular cells (HFRTC) were cultured in vitro. HFRTC infected or not infected by HFRSV were observed by using trypan-blue stain and transmission electron microscopy(TEM) . Viral-mRNA was detected by in situ molecular hybridization. Results (1)HFRSV could directly infected HFRTC; (2)The death rate of HFRTC in the infection group was significantly higher than that in the control group 1 to 4 weeks after infection; (3) Injuries of cell membrane and cell organs after infection with HFRSV were significantly earlier and more severe as compared to control by means of TEM. Conclusion HFRSV can directly damage renal tubular cells(RTC), which contributes to the pathogenesis of hemorrhagic fever with renal syndrome(HFRS).
, http://www.100md.com
Keywords：Hemorrhagic fever; Renal syndrome; Renal tubular cells; Hemorrhagic fever virus
基金项目：卫生部资助基金项目(96-1-142)
参考文献：
[1]周柱亮，韩敬明，汪建国，等.肾综出血热患者肾病理与临床分析.中华传染病杂志，1996，14：237-238.
[2]邵佑之.兔肾近端小管上皮细胞的培养.解剖学报， 1991，22：297-300.
[3]姜泊，张亚历，周殿元，等.分子生物学常用实验方法.第2版.北京：人民军医出版社，1996.137-139.
[4]Aled O, Philis RS, Topley N, et al. Elevated d-glucose concentrations modulate TGF-β1 systhesis by human cultured renal proximal tubular cells. Am J Pathol, 1995, 147: 362-373.
, http://www.100md.com
[5]洪涛.从肾综出血热综合征急性期患者尸体肾组织中发现的病毒相关形态.中华实验和临床病毒杂志，1990，68：381-383.
[6]杨守京，刘彦仿，胡锡瑞，等.肾综出血热肾组织中病毒感染及病毒抗原的来源和意义.第四军医大学学报，1995，16：401-408.
[7]Racusen LC, Fivush BA, Li YL, et al. Dissociation of tubular cell detachment and tubular cell death in clinical and experimental "acute tubular necrosis". Lab Inv, 1991, 64: 546-555.
收稿日期：1999-09-23, http://www.100md.com
单位：430022武汉，同济医科大学附属协和医院肾内科(E-mail：yaoli@Public.wh.hb.cn)
关键词：出血热；肾综合征；肾小管上皮细胞；出血热病毒
中华肾脏病杂志000309摘 要：目的研究肾综合征出血热病毒(HFRSV)对肾小管上皮细胞(RTC)的致病作用。方法应用HFRSV感染体外培养的人胚RTC(HFRTC)，采用原位分子杂交技术检测HFRTC内的HFRSV并作台盼蓝染色、透射电镜(TEM)观察。结果(1)HPRSV能够直接感染培养的HFRTC；(2)从第7天起被感染的HPRTC死亡率明显高于对照组；(3)TEM观察表明HFRSV可致HPRTC的细胞膜、细胞器损害。结论HFRSV能够直接损害体外培养的HPRTC，其对RTC的损害作用可能直接参予肾综出血热(HPRS)的发病。
, 百拇医药
The study of cytopathogenic effect of hemorrhagic fever with renal syndrome virus on renal tubular cells
YAO Lijun,DENG Anguo,HUANG Pei
(Department of Nephrology, Union Hospital , Tongji Medical University, Wuhan 430022, China)
Abstract：Objective To study the cytopathogcnic effect of epidemic hemorrhagic fever with renal syndrome virus (HFRSV) on renal tubular cells(RTC) . Methods Human fetal renal tubular cells (HFRTC) were cultured in vitro. HFRTC infected or not infected by HFRSV were observed by using trypan-blue stain and transmission electron microscopy(TEM) . Viral-mRNA was detected by in situ molecular hybridization. Results (1)HFRSV could directly infected HFRTC; (2)The death rate of HFRTC in the infection group was significantly higher than that in the control group 1 to 4 weeks after infection; (3) Injuries of cell membrane and cell organs after infection with HFRSV were significantly earlier and more severe as compared to control by means of TEM. Conclusion HFRSV can directly damage renal tubular cells(RTC), which contributes to the pathogenesis of hemorrhagic fever with renal syndrome(HFRS).
, http://www.100md.com
Keywords：Hemorrhagic fever; Renal syndrome; Renal tubular cells; Hemorrhagic fever virus
基金项目：卫生部资助基金项目(96-1-142)
参考文献：
[1]周柱亮，韩敬明，汪建国，等.肾综出血热患者肾病理与临床分析.中华传染病杂志，1996，14：237-238.
[2]邵佑之.兔肾近端小管上皮细胞的培养.解剖学报， 1991，22：297-300.
[3]姜泊，张亚历，周殿元，等.分子生物学常用实验方法.第2版.北京：人民军医出版社，1996.137-139.
[4]Aled O, Philis RS, Topley N, et al. Elevated d-glucose concentrations modulate TGF-β1 systhesis by human cultured renal proximal tubular cells. Am J Pathol, 1995, 147: 362-373.
, http://www.100md.com
[5]洪涛.从肾综出血热综合征急性期患者尸体肾组织中发现的病毒相关形态.中华实验和临床病毒杂志，1990，68：381-383.
[6]杨守京，刘彦仿，胡锡瑞，等.肾综出血热肾组织中病毒感染及病毒抗原的来源和意义.第四军医大学学报，1995，16：401-408.
[7]Racusen LC, Fivush BA, Li YL, et al. Dissociation of tubular cell detachment and tubular cell death in clinical and experimental "acute tubular necrosis". Lab Inv, 1991, 64: 546-555.
收稿日期：1999-09-23, http://www.100md.com