HPLC法测定体液中氨溴索浓度及其药代动力学参数
作者：张立超 胡晋红 李珍 宋洪杰
单位：张立超 胡晋红 李珍 宋洪杰(第二军医大学附属长海医院药学部 上海 200433)
关键词：盐酸氨溴索，血药浓度；尿药浓度，高效液相色谱法
西北药学杂志000312摘 要：建立了HPLC测定人血浆及尿中盐酸氨溴索含量的方法；HypersillC18柱(4.6mm×250mm，5μm)，乙腈-甲醇-0.Olmol/L磷酸盐缓冲液-四氢呋喃(35:35:27.5:2.5,V/V)为流动相，流速1.5ml/min，检测波长242nm。结果表明；最低检测浓度为5ng/ml，血药浓度在10～320ng/ml范围内线性良好，尿药浓度在0.25～8.0μg/ml范围内线性良好，氨溴索生物半衰期为(4.21±0.93)h。
分类号：R969.1 文献标识码：A
Determination of ambroxol hydrochloride in human plasma and urine and pharmacokinetic parameter by HPLC
Zhang Lichao ,Hu Jinhong ,Li Zhen ,Song Hongjie
(Changhai Hospital，Second Military Medical University,Shanghai 200433)
Abstract：An HPLC method of determination of ambroxol hydrochloride in human plasma and urine was established.The analytical column was Hypersil C18(4.6mm×250mm，5μm).The mobile phase was the mixture of acetonitrile-methanol-0.01mol/L phosphate buffer-tetrahydrofuran(35:35:27.5:2.5,V/V).The flow rate was 1.5ml/min.The derector set at 242nm.The linear range of plasma concentration was 10～320ng/ml，that of urine concentration was 0.25～8.Oμg/ml.A single dose of ambroxol hydrochloride capsule was taken orally by eight healthy volunteers.The level of anbroxol in plasma and urine was determined with this method.The results showed the biological half life of ambroxol was 4.2lh.
Keywords：ambroxol hydrochloridel plasma concentration; urine concentration;HPLC
参考文献：
[1]Botterblom MH, Janssen TJ, Guelen PJ. Rapid and sensitive determination of ambroxol in human plasma and urine by high-performance liquid chromatography. J Chromatogr, 1987,421(1):211
[2]Flores-Murrieta FJ, Hoyo-Vadillo C, Hong E, et al.Assay of ambroxol in human plasma by highperformance liquid chromatography with amperometris detection. J Chromatogr Biomed Appl, 1989,490(2):464
[3]Schmid J. Assay of ambroxol in biological fluids by capillary gas-liquid chromatography. J Chromatogr,1987,414(1):65
收稿日期：1999-12-09, http://www.100md.com
单位：张立超 胡晋红 李珍 宋洪杰(第二军医大学附属长海医院药学部 上海 200433)
关键词：盐酸氨溴索，血药浓度；尿药浓度，高效液相色谱法
西北药学杂志000312摘 要：建立了HPLC测定人血浆及尿中盐酸氨溴索含量的方法；HypersillC18柱(4.6mm×250mm，5μm)，乙腈-甲醇-0.Olmol/L磷酸盐缓冲液-四氢呋喃(35:35:27.5:2.5,V/V)为流动相，流速1.5ml/min，检测波长242nm。结果表明；最低检测浓度为5ng/ml，血药浓度在10～320ng/ml范围内线性良好，尿药浓度在0.25～8.0μg/ml范围内线性良好，氨溴索生物半衰期为(4.21±0.93)h。
分类号：R969.1 文献标识码：A
Determination of ambroxol hydrochloride in human plasma and urine and pharmacokinetic parameter by HPLC
Zhang Lichao ,Hu Jinhong ,Li Zhen ,Song Hongjie
(Changhai Hospital，Second Military Medical University,Shanghai 200433)
Abstract：An HPLC method of determination of ambroxol hydrochloride in human plasma and urine was established.The analytical column was Hypersil C18(4.6mm×250mm，5μm).The mobile phase was the mixture of acetonitrile-methanol-0.01mol/L phosphate buffer-tetrahydrofuran(35:35:27.5:2.5,V/V).The flow rate was 1.5ml/min.The derector set at 242nm.The linear range of plasma concentration was 10～320ng/ml，that of urine concentration was 0.25～8.Oμg/ml.A single dose of ambroxol hydrochloride capsule was taken orally by eight healthy volunteers.The level of anbroxol in plasma and urine was determined with this method.The results showed the biological half life of ambroxol was 4.2lh.
Keywords：ambroxol hydrochloridel plasma concentration; urine concentration;HPLC
参考文献：
[1]Botterblom MH, Janssen TJ, Guelen PJ. Rapid and sensitive determination of ambroxol in human plasma and urine by high-performance liquid chromatography. J Chromatogr, 1987,421(1):211
[2]Flores-Murrieta FJ, Hoyo-Vadillo C, Hong E, et al.Assay of ambroxol in human plasma by highperformance liquid chromatography with amperometris detection. J Chromatogr Biomed Appl, 1989,490(2):464
[3]Schmid J. Assay of ambroxol in biological fluids by capillary gas-liquid chromatography. J Chromatogr,1987,414(1):65
收稿日期：1999-12-09, http://www.100md.com