ICAM-1 和 E-selectin 在急性胆管炎肝损害中的作用
作者：黄显凯 韩本立
单位：黄显凯(第三军医大学大坪医院普外科(400042))；韩本立(第三军医大学西南医院肝胆中心)
关键词：胆道感染；中性粒细胞；肝损害
重庆医学000408摘 要：目的探讨ICAM-1和E-selectim在急性胆管炎肝损害中的作用。方法应用组织学方法观察ICAM-1和E-selectin对肝组织PMN浸润及其肝损害作用的影响。结果急性胆管炎时PMN在肝窦及肝细胞周围大量聚集，大鼠肝细胞变性坏死，动物12小时存活率仅为56.6%。抗ICAN-1及E-selectin单抗预处理使肝组织PMN数量明显减少，肝坏死面积减少，动物存活率提高。结果ICAM-1和E-selectin在胆道感染时肝组织PMN浸润及其肝损害过程中发挥重要作用。
Effcts of ICAM-1 and E-selectin on Hepatic Damage in Severe Cholangitis
Huang Xiankai ,Hang Benli
(Department of General Sugery, Daping Hospital, Third Military Medical University, Chongqing 400042)
Abstract：AimH&Methods:The effects of ICAM-1 and E-selectin on PMN accumulation in liver and hepatic damage were investigated.Results: It was found that PMN accumulated in hepatic sinusoids and around hepatocytes, necrosis of the hepatocytes took place, and only 66.6 % of rats survived 12 hours after infection. Pretreatment of anti ICAM-1 and E-selectin mAb decreased the number of PMN in hepatic sinusoid and near hepattocytes, decreased necrosis of the hepatocytes, and improved survival rate of the infected rates. Conclusion: The results suggest that ICAM -1 and E-selectin play an important role in PMN accumulation and hepatic damage in acute cholangitis.
Keywords：Cholangitis; Polymorphonuclear cell; Hepatic damage
参考文献：
[1]Komatsu Y, Shiratori Y, Kawase T, et al. Role of polymorphonuclear leukocytes in galactosamine hepatitis mechanism of adherence to hepatic endothelial cell Hepatology, 1994, 20:1548
[2]Essani NA, Fisher MA, Farhood A, et al. Cytokine-induced up regulation of hepatic irgexcellualar adhesion rrolecule-lmessenger RNA expression and its role in the pathophysiology of murine endotoxin shock and acute liver failure. Hepatology, 1995, 21:1632
[3]金伯朱 主编.细胞和分子免疫学.世界图书出版公司. 1994
[4]Miller VM, Komori K, Burnett JC, et al. Differential sensitivity to endothelin on canine arteries and veins. Am J Pysiol, 1989, 257:Hl127
[5]王迪 主编. 病理生理学. 北京:人民卫生出版社, 1994:59, 百拇医药
单位：黄显凯(第三军医大学大坪医院普外科(400042))；韩本立(第三军医大学西南医院肝胆中心)
关键词：胆道感染；中性粒细胞；肝损害
重庆医学000408摘 要：目的探讨ICAM-1和E-selectim在急性胆管炎肝损害中的作用。方法应用组织学方法观察ICAM-1和E-selectin对肝组织PMN浸润及其肝损害作用的影响。结果急性胆管炎时PMN在肝窦及肝细胞周围大量聚集，大鼠肝细胞变性坏死，动物12小时存活率仅为56.6%。抗ICAN-1及E-selectin单抗预处理使肝组织PMN数量明显减少，肝坏死面积减少，动物存活率提高。结果ICAM-1和E-selectin在胆道感染时肝组织PMN浸润及其肝损害过程中发挥重要作用。
Effcts of ICAM-1 and E-selectin on Hepatic Damage in Severe Cholangitis
Huang Xiankai ,Hang Benli
(Department of General Sugery, Daping Hospital, Third Military Medical University, Chongqing 400042)
Abstract：AimH&Methods:The effects of ICAM-1 and E-selectin on PMN accumulation in liver and hepatic damage were investigated.Results: It was found that PMN accumulated in hepatic sinusoids and around hepatocytes, necrosis of the hepatocytes took place, and only 66.6 % of rats survived 12 hours after infection. Pretreatment of anti ICAM-1 and E-selectin mAb decreased the number of PMN in hepatic sinusoid and near hepattocytes, decreased necrosis of the hepatocytes, and improved survival rate of the infected rates. Conclusion: The results suggest that ICAM -1 and E-selectin play an important role in PMN accumulation and hepatic damage in acute cholangitis.
Keywords：Cholangitis; Polymorphonuclear cell; Hepatic damage
参考文献：
[1]Komatsu Y, Shiratori Y, Kawase T, et al. Role of polymorphonuclear leukocytes in galactosamine hepatitis mechanism of adherence to hepatic endothelial cell Hepatology, 1994, 20:1548
[2]Essani NA, Fisher MA, Farhood A, et al. Cytokine-induced up regulation of hepatic irgexcellualar adhesion rrolecule-lmessenger RNA expression and its role in the pathophysiology of murine endotoxin shock and acute liver failure. Hepatology, 1995, 21:1632
[3]金伯朱 主编.细胞和分子免疫学.世界图书出版公司. 1994
[4]Miller VM, Komori K, Burnett JC, et al. Differential sensitivity to endothelin on canine arteries and veins. Am J Pysiol, 1989, 257:Hl127
[5]王迪 主编. 病理生理学. 北京:人民卫生出版社, 1994:59, 百拇医药