突触体素、S-100蛋白、NSE免疫反应神经纤维在人脾脏的分布
作者：吕世军 刘雨清 张伟栋 张式暖 郭爱华 孙永红 吴洪娟 黄岩
单位：潍坊医学院病理学教研室，组织学与胚胎学教研室，潍坊 261042
关键词：突触体素；S-100蛋白；NSE；免疫组织化学；脾脏；人
解剖科学进展000426 摘要 探讨人脾脏突触体素、S-100蛋白和NSE免疫反应神经纤维的支配，为脾脏的神经免疫相互作用提供形态学资料。应用免疫组织化学ABC法观察正常人脾脏30例，福尔马林固定，石蜡包埋组织切片。结果显示突触体素、S-100蛋白和NSE免疫反应神经纤维呈细丝、串珠状，沿着脾血管的各级分支进入脾实质，主要沿着脾动脉的分支而分布，以中央动脉血管尤为丰富，它们分布在白髓、红髓和边缘区，穿行于淋巴细胞之间。人脾脏小梁中未查见免疫反应神经纤维。由此可提示人类脾脏有突触体素、S-100蛋白、NSE免疫反应神经纤维的分布，为人类脾脏神经免疫相互作用提供了形态学资料。
, 百拇医药
The Distribution of Synaptophysin, S-100 Protein, NSE Immunoreactive Nerve Fibers in Human Spleen
Lu Shijun, Liu Yuqing, Zhang Weidong, et al
(Depargment of Pathology, Weifang Medical College, WeiFang 261042)
Abstract To study the distribution of synaptophysin, S-100 protein and neuronal specific enolase (NSE) immunoreactive nerve fibers in human spleen, supply morphologic data for the interactions between nerve and immunity in human spleen. 30 samples of normal human spleen were fixed in 10% formalin, embeded in paraffin and stained by immunohistochemical ABC method. In human spleen, synaptophysin, S-100 protein and NSE immunoreactive nerve fibers were observed to be presented as linear, varicose profiles, entering the parenchyma with the vasculature, traveling along the branching vasculature and distributing mainly in the central artery with vascular plexus. In the white pulp, red pulp and marginal zone there are immunoreactive nerve fibers and close to lymphocytes. Immuhnoreactive nerve fibers were not found in the speen trabecula. It is suggested that there are innervation of synaptophysin, S-100 protein and NSE immunoreactive nerve fibers in human spleen which might provide morphologic data for the interactions between nerve and immunity in human spleen.
, http://www.100md.com
Key words synaptophysin; S-100 protein; NSE; human; spleen
山东省教委资助课题
参考文献
1，Payan DG, Mcgillis JP, Goetzl EJ. Neuroimmunology. Advance in immunology, 1986,39:299
2，范少光，丁桂风.神经内分泌与免疫系统之间相互作用的介导物质：共用的生物学语言.生理科学进展，1995，26(2)：175
3，邱一华，王健鹤.神经递质和神经肽对免疫功能的调节.生理科学进展，1991，22(1)：21
4，Goetzl EJ, Xia M, Ingram DA, et al. Neuropeptide signaling of lymphocytes in immunological response. Int Arch Allergy Immunol, 1995,107:202
, http://www.100md.com
5， Lunderg JM, Anggard A, Pernow J, et al. Neuropeptide Y, Substance P, VIP. Immunoreactive nerves in cat spleen in relation to autonomic vascular and volume control, Cell Tissue Res, 1985,239(1):9
6， Olschowka JA, Felten SY, Bellinger DL, et al. NPY-positive nerve terminals contact lymphocytes in the rat periarteriolar lymphatic shelth of the rat splenic white pulp. Soc Neurosci Abstr Washington, 1988,14(1):1280
7， Romano TA, Olschowka JA, Felten SY, et al. Neuropeptide-Y involvment in neural-immune interreactions in the rat spleen. Soc Neurosci Abstr Washington, 1989,15(1):714
, http://www.100md.com
8，吴中亮，张远强，苏慧慈.神经肽Y免疫反应性神经纤维在大鼠脾脏内的分布.解剖学报，1993，24(1)：27
9，杨宏，邱建勇，鞠躬.GAP-43免疫反应神经纤维在小鼠脾脏的分布及其性质的研究.神经解剖学杂志，1997，13(1)：6
10，Felten DL, Felten SY, Carson SL, et al. Noradrenergic and peptidergic innervation of lymphoid tissue. J Immunology, 1985,135(2):755
11，杜红燕，吴馥梅.突触素Ⅰ的若干研究进展.生物化学与生物物理进展，1995，22(5)：422, http://www.100md.com
单位：潍坊医学院病理学教研室，组织学与胚胎学教研室，潍坊 261042
关键词：突触体素；S-100蛋白；NSE；免疫组织化学；脾脏；人
解剖科学进展000426 摘要 探讨人脾脏突触体素、S-100蛋白和NSE免疫反应神经纤维的支配，为脾脏的神经免疫相互作用提供形态学资料。应用免疫组织化学ABC法观察正常人脾脏30例，福尔马林固定，石蜡包埋组织切片。结果显示突触体素、S-100蛋白和NSE免疫反应神经纤维呈细丝、串珠状，沿着脾血管的各级分支进入脾实质，主要沿着脾动脉的分支而分布，以中央动脉血管尤为丰富，它们分布在白髓、红髓和边缘区，穿行于淋巴细胞之间。人脾脏小梁中未查见免疫反应神经纤维。由此可提示人类脾脏有突触体素、S-100蛋白、NSE免疫反应神经纤维的分布，为人类脾脏神经免疫相互作用提供了形态学资料。
, 百拇医药
The Distribution of Synaptophysin, S-100 Protein, NSE Immunoreactive Nerve Fibers in Human Spleen
Lu Shijun, Liu Yuqing, Zhang Weidong, et al
(Depargment of Pathology, Weifang Medical College, WeiFang 261042)
Abstract To study the distribution of synaptophysin, S-100 protein and neuronal specific enolase (NSE) immunoreactive nerve fibers in human spleen, supply morphologic data for the interactions between nerve and immunity in human spleen. 30 samples of normal human spleen were fixed in 10% formalin, embeded in paraffin and stained by immunohistochemical ABC method. In human spleen, synaptophysin, S-100 protein and NSE immunoreactive nerve fibers were observed to be presented as linear, varicose profiles, entering the parenchyma with the vasculature, traveling along the branching vasculature and distributing mainly in the central artery with vascular plexus. In the white pulp, red pulp and marginal zone there are immunoreactive nerve fibers and close to lymphocytes. Immuhnoreactive nerve fibers were not found in the speen trabecula. It is suggested that there are innervation of synaptophysin, S-100 protein and NSE immunoreactive nerve fibers in human spleen which might provide morphologic data for the interactions between nerve and immunity in human spleen.
, http://www.100md.com
Key words synaptophysin; S-100 protein; NSE; human; spleen
山东省教委资助课题
参考文献
1，Payan DG, Mcgillis JP, Goetzl EJ. Neuroimmunology. Advance in immunology, 1986,39:299
2，范少光，丁桂风.神经内分泌与免疫系统之间相互作用的介导物质：共用的生物学语言.生理科学进展，1995，26(2)：175
3，邱一华，王健鹤.神经递质和神经肽对免疫功能的调节.生理科学进展，1991，22(1)：21
4，Goetzl EJ, Xia M, Ingram DA, et al. Neuropeptide signaling of lymphocytes in immunological response. Int Arch Allergy Immunol, 1995,107:202
, http://www.100md.com
5， Lunderg JM, Anggard A, Pernow J, et al. Neuropeptide Y, Substance P, VIP. Immunoreactive nerves in cat spleen in relation to autonomic vascular and volume control, Cell Tissue Res, 1985,239(1):9
6， Olschowka JA, Felten SY, Bellinger DL, et al. NPY-positive nerve terminals contact lymphocytes in the rat periarteriolar lymphatic shelth of the rat splenic white pulp. Soc Neurosci Abstr Washington, 1988,14(1):1280
7， Romano TA, Olschowka JA, Felten SY, et al. Neuropeptide-Y involvment in neural-immune interreactions in the rat spleen. Soc Neurosci Abstr Washington, 1989,15(1):714
, http://www.100md.com
8，吴中亮，张远强，苏慧慈.神经肽Y免疫反应性神经纤维在大鼠脾脏内的分布.解剖学报，1993，24(1)：27
9，杨宏，邱建勇，鞠躬.GAP-43免疫反应神经纤维在小鼠脾脏的分布及其性质的研究.神经解剖学杂志，1997，13(1)：6
10，Felten DL, Felten SY, Carson SL, et al. Noradrenergic and peptidergic innervation of lymphoid tissue. J Immunology, 1985,135(2):755
11，杜红燕，吴馥梅.突触素Ⅰ的若干研究进展.生物化学与生物物理进展，1995，22(5)：422, http://www.100md.com