小鼠颌骨发育过程中DMP1基因相关lncRNA的表达研究(1)
【摘要】 目的 研究C57小鼠不同发育时期颌骨中DMP1基因附近长链非编码RNA(lncRNA)的表达变化情况。 方法 利用lncRNA測序技术和实时定量PCR(RT?PCR)检测孕18 d(E18D)及出生后2周(2W)的C57小鼠下颌骨组织样本中DMP1基因位置附近的lncRNA表达变化，分析其与DMP1基因表达变化的关系。 结果 E18D组与2W组间有6 617条差异表达的lncRNA(∣log2 (2W/E18D)∣>1且控制错误发现率<0.01)，其中有5条lncRNA在DMP1基因上游或下游300 000 bp以内。RT?PCR分析显示，2W组中DMP1和lnc19450的表达较E18D组降低，而lnc30219、lnc8292、lnc30219和lnc32865的表达较E18D组上升(P均<0.05)。结论 lnc19450的表达与DMP1基因的表达趋势一致，lnc30219、lnc8292、lnc30219和lnc32865的表达与DMP1基因的表达趋势相反，推测lnc19450、 lnc30219、lnc8292、lnc30219和lnc32865可能在DMP1基因的表达调控起着重要的作用。
【关键词】 长链非编码核糖核酸;牙本质基质蛋白1基因;小鼠颌骨发育
【Abstract】 Objective To analyze the changes in the expression of long non?coding RNA (1ncRNA) adjacent to the DMP1 gene in different developmental stages of jawbone in C57 mice. Methods The lncRNA sequencing and quantitative real?time PCR (RT?PCR) were performed to detect the expression profile of DMP1 gene?related lncRNAs at the embryonic day 18 (E18D) and 2 weeks after birth (2W) in the jawbone of C57 mice，and analyze the relationship between lncRNA expression and the changes in the expression of DMP1. Results Compared with the E18D group，6 617 lncRNAs were differentially expressed in the 2W group (∣log2 (2W/E18D)∣>1 and FDR<0.01)，among which，5 lncRNAs were at the upstream or downstream of DMP1 gene position within 300 kb. RT?PCRdemonstrated that the expression levels of DMP1 and lnc19450 in the 2W group were lower than those in the E18D group，whereas the expression levels of lnc30219，lnc8292，lnc30219 and lnc32865 were higher compared with those in the E18D group (all P<0.05). Conclusions The expression trends of lnc19450 and DMP1 are consistent，whereas the expression patterns of lnc30219，lnc8292，lnc30219 and lnc32865 are opposite with that of DMP1 gene，suggesting that lnc19450，lnc30219，lnc8292，lnc30219 and lnc32865 may play a vital role in regulating the expression of DMP1 gene.
【Key words】 Long non?coding RNA;DMP1 gene;Mouse jawbone development
长链非编码RNA(lncRNA)是一类转录本长度超过200 nt的RNA分子，不编码蛋白，在生物体重要生命活动中发挥着极广泛的调控作用[1?3]。lncRNA 的功能多样，最重要的作用是参与基因表达的调节。目前研究表明，lncRNA可以在启动子区通过顺式作用元件(启动子或增强子)或反式作用因子(各种DNA结合因子)调控相关基因的表达[4?5]。牙本质基质蛋白1(DMP1)是骨和牙本质特异性蛋白，在牙和颌骨的分化、成熟过程以及正确调节骨和牙本质的矿化中具有复杂而重要的作用[6?7]。DMP1基因的正常表达是骨和牙齿正常发育、钙化、干细胞向骨和牙本质分化以及正常的矿物质代谢的基础[8?9]。因此，研究DMP1基因表达调控的变化具有重要意义。本研究利用lncRNA测序技术和实时定量PCR(RT?PCR)检测孕18 d(E18D)及出生后2周(2W)的C57小鼠下颌骨组织样本中DMP1基因位置附近的lncRNA的表达变化，并分析它们与DMP1基因表达的趋势。, 百拇医药(夏昕 阮毅 陈绛媛 孔祥波 伍虹)
【关键词】 长链非编码核糖核酸;牙本质基质蛋白1基因;小鼠颌骨发育
【Abstract】 Objective To analyze the changes in the expression of long non?coding RNA (1ncRNA) adjacent to the DMP1 gene in different developmental stages of jawbone in C57 mice. Methods The lncRNA sequencing and quantitative real?time PCR (RT?PCR) were performed to detect the expression profile of DMP1 gene?related lncRNAs at the embryonic day 18 (E18D) and 2 weeks after birth (2W) in the jawbone of C57 mice，and analyze the relationship between lncRNA expression and the changes in the expression of DMP1. Results Compared with the E18D group，6 617 lncRNAs were differentially expressed in the 2W group (∣log2 (2W/E18D)∣>1 and FDR<0.01)，among which，5 lncRNAs were at the upstream or downstream of DMP1 gene position within 300 kb. RT?PCRdemonstrated that the expression levels of DMP1 and lnc19450 in the 2W group were lower than those in the E18D group，whereas the expression levels of lnc30219，lnc8292，lnc30219 and lnc32865 were higher compared with those in the E18D group (all P<0.05). Conclusions The expression trends of lnc19450 and DMP1 are consistent，whereas the expression patterns of lnc30219，lnc8292，lnc30219 and lnc32865 are opposite with that of DMP1 gene，suggesting that lnc19450，lnc30219，lnc8292，lnc30219 and lnc32865 may play a vital role in regulating the expression of DMP1 gene.
【Key words】 Long non?coding RNA;DMP1 gene;Mouse jawbone development
长链非编码RNA(lncRNA)是一类转录本长度超过200 nt的RNA分子，不编码蛋白，在生物体重要生命活动中发挥着极广泛的调控作用[1?3]。lncRNA 的功能多样，最重要的作用是参与基因表达的调节。目前研究表明，lncRNA可以在启动子区通过顺式作用元件(启动子或增强子)或反式作用因子(各种DNA结合因子)调控相关基因的表达[4?5]。牙本质基质蛋白1(DMP1)是骨和牙本质特异性蛋白，在牙和颌骨的分化、成熟过程以及正确调节骨和牙本质的矿化中具有复杂而重要的作用[6?7]。DMP1基因的正常表达是骨和牙齿正常发育、钙化、干细胞向骨和牙本质分化以及正常的矿物质代谢的基础[8?9]。因此，研究DMP1基因表达调控的变化具有重要意义。本研究利用lncRNA测序技术和实时定量PCR(RT?PCR)检测孕18 d(E18D)及出生后2周(2W)的C57小鼠下颌骨组织样本中DMP1基因位置附近的lncRNA的表达变化，并分析它们与DMP1基因表达的趋势。, 百拇医药(夏昕 阮毅 陈绛媛 孔祥波 伍虹)