祝彩霞?杨娟?袁林静?牛刚

    【摘要】目的 檢测膜突蛋白(MSN)在进展型高级别浆液性卵巢癌(HGSOC)中的表达情况，分析MSN表达上调对卵巢腺癌细胞增殖和侵袭能力的影响。方法 在124例进展型HGSOC标本中应用逆转录PCR检测MSN mRNA的表达情况，分析其与临床特征和预后的关系。体外培养卵巢腺癌细胞OVMZ-6，转染过表达MSN后应用逆转录PCR和蛋白免疫印迹法验证MSN表达变化;分别采用四甲基偶氮唑蓝(MTT)法、Transwell小室实验和流式细胞仪检测MSN表达上调后对卵巢腺癌细胞增殖和侵袭能力的影响。结果 进展型HGSOC标本均能检出MSN mRNA的表达，Kaplan-Meier生存分析显示MSN mRNA高表达者总生存期高于MSN mRNA低表达者(P 0.05)。多因素Cox回归分析显示MSN mRNA高表达是总生存期的独立保护因素(HR = 0.51，95%CI 0.31 ～ 0.84，P = 0.009)。MSN-pRc/RSV转染后，OVMZ-6细胞中MSN mRNA和蛋白表达水平显著上调。MSN过表达的OVMZ-6细胞增殖能力和侵袭能力明显下降(P均 0.05). Multivariate Coxs regression analysis demonstrated increased MSN mRNA expression was identified as an independent favorable prognostic factor for OS (HR = 0.51，95%CI 0.31 ～ 0.84，P = 0.009). The expression levels of MSN mRNA and protein were significantly up-regulated after transfection with MSN-pRc/RSV. The cell proliferation and invasiveness were significantly inhibited in ovarian cancer cells transfected with MSN overexpression (all P 0.05) ......