林志玲?黎洁瑶?闵筱辉?许稷豪?于涛?陈其奎

    【摘要】目的 分析高糖狀态对结肠肿瘤细胞增殖的影响，并探讨Wnt抑制因子1(WIF1)通过Wnt/β-连环蛋白(β-catenin)通路影响高糖状态下结肠肿瘤细胞增殖的机制。方法 用不同浓度的D-葡萄糖处理结肠肿瘤细胞株SW620细胞，通过实时荧光定量PCR(qPCR)和蛋白免疫印迹法测定增殖相关基因的表达，行细胞增殖活性检测、细胞计数实验，用细胞平板克隆形成实验检测细胞增殖情况，采用qPCR和蛋白免疫印迹法测定WIF1和β-catenin的表达。转染siRNA和过表达质粒调控WIF1的表达后，检测WIF1对SW620细胞增殖能力和β-catenin表达水平的影响。结果 随着糖浓度的增加，SW620细胞的增殖能力增强(P均< 0.05)，WIF1的表达下降而β-catenin的表达增高(P均< 0.05)。下调WIF1的表达，SW620细胞的增殖能力增强且β-catenin的表达增高(P均< 0.05)，而过表达WIF1后，SW620细胞的增殖能力受到抑制且β-catenin的表达下降(P均< 0.05)。结论 高糖状态下WIF1表达的下降，可能通过活化Wnt/β-catenin通路促进高糖状态下结肠肿瘤细胞的增殖。

    【关键词】高糖;结肠肿瘤;Wnt抑制因子1;β-连环蛋白;增殖

    High glucose regulates the proliferation of colon cancer cells through the WIF1-Wnt/β-catenin signaling pathway Lin Zhiling， Li Jieyao， Min Xiaohui， Xu Jihao， Yu Tao， Chen Qikui. Department of Gastroenterology， Sun Yat-sen Memorial Hospital of Sun Yat-sen University， Guangzhou 510120， China

    Corresponding author， Chen Qikui， E-mail： qkchen2015@ 163. com

    【Abstract】Objective To evaluate the effect of high glucose on the proliferation of colon cancer cells， and investigate the mechanism of the effect of Wnt inhibitory factor 1 (WIF1) on the proliferation of colon cancer cells under high glucose state through the Wnt/β-catenin signaling pathway. Methods Colon cancer cell line SW620 cells were treated with different doses of D-glucose. The expressions of proliferation-associated mRNA and protein were measured by real-time fluorescent quantitative PCR (qPCR) and Western blot. The proliferation ability of SW620 cells was measured by cell proliferation assay ......