梁沛彰?颜茹芳?张永华?黄焕森?汪灵芝

    【摘要】目的 探讨3种不同机制抗抑郁药对海马星形胶质细胞缝隙连接蛋白和通道功能的影响。方法 CCK-8实验检测不同机制抗抑郁药对海马星形胶质细胞的细胞毒性，采用蛋白免疫印迹法和细胞接种荧光示踪法测定3种不同机制抗抑郁药对由缝隙连接蛋白43(Cx43)组成的通道功能和Cx43蛋白表达的影响。结果 CCK-8实验显示，25.00 ?mol/L氟西汀、0.10 ?mol/L阿米替林和0.20 nmol/L文拉法辛对海马星形胶质细胞无细胞毒性(P均> 0.05); 25.00 ?mol/L氟西汀、0.10 ?mol/L阿米替林和0.20 nmol/L文拉法辛能抑制海马星形胶质细胞缝隙连接的荧光传递功能(P均 0.05)。结论 抗抑郁药能够显著降低海马星形胶质细胞Cx43组成的通道功能。

    【关键词】细胞缝隙连接;星形胶质细胞;海马;抗抑郁药;缝隙连接蛋白43

    Influence of antidepressants on connexin expression and gap junction function of hippocampal astrocytes Liang Peizhang， Yan Rufang， Zhang Yonghua， Huang Huansen， Wang Lingzhi. Department of Anesthesiology， the Second Affiliated Hospital of Guangzhou Medical University， Guangzhou 510260， China

    Corresponding author， Wang Lingzhi， E-mail： wlz4009@163.com

    【Abstract】Objective To evaluate the effects of three antidepressants with different mechanisms on connexin expression and channel function of hippocampal astrocytes. Methods The cytotoxicity of antidepressants with different mechanisms on hippocampal astrocytes was assessed by CCK-8 assay. The effects of three antidepressants with different mechanisms on gap junction function and the expression level of connexin 43 protein (Cx43) were determined by cell inoculation fluorescence tracing and western blot. Results CCK-8 assay showed that 25.00 ?mol/L fluoxetine ......