【摘要】 目的 探索Rictor/哺乳动物雷帕霉素靶蛋白复合体2(Rictor/mTORC2)信号在Kras突变的肿瘤细胞中的表达情况及对癌细胞增殖和致瘤性的影响。方法 利用公共数据库的泛癌种数据了解Kras和Rictor基因变异的频率。通过Pearson相关性分析研究Kras和Rictor基因变异和基因表达的共表达趋势。利用Kras突变的多种癌症细胞模型，通过反义RNA序列下调Rictor基因的表达，分别通过CCK-8、克隆形成实验了解Rictor/mTORC2信号通路对Kras突变的肿瘤细胞的增殖和致瘤性的影响。结果 公共数据库分析显示Kras和Rictor基因的变异频率分别超过90%及30%，最常见于胰腺癌、肺癌、结肠癌和乳腺癌。一系列携带Kras基因突变以及Kras基因野生型的细胞株Rictor基因检测结果中，Pearson相关性分析显示Kras和Rictor基因变异呈正相关(均P lt; 0.05)，mRNA表达水平存在共同变化趋势；下调Rictor基因的表达可抑制Kras突变的癌细胞的增殖和致瘤能力(均P lt; 0.05)。结论 Rictor/mTORC2信号通路在Kras突变的癌细胞中明显活化，并可促进Kras突变的癌细胞的增殖和致瘤性。

    【关键词】 Kras；Rictor；Kras突变的癌细胞；增殖；致瘤性

    Rictor/mTORC2 signaling promotes proliferation and tumorigenicity of Kras mutant cancer cells

    LAI Huiling， CHEN Shuqin

    (Department of Obstetrics and Gynecology， the Sixth Affiliated Hospital of Sun Yat-sen University， Guangzhou 510655， China)

    Corresponding author， CHEN Shuqin， E-mail： chshqin@mail.sysu.edu.cn

    【Abstract】 Objective To explore the expression profile of Rictor/mTORC2 in Kras mutant tumor cells and its effect on cell proliferation and tumorigenicity. Methods The frequency of Kras and Rictor gene variations was investigated by using pan-cancer data from public databases. The co-expression relationship between Kras and Rictor gene variations and their respective gene expressions was analyzed using Pearson correlation analysis. Multiple cancer cell models with Kras mutation were used to down-regulate the expression of Rictor gene through antisense RNA sequence ......