富血小板血浆对人成骨样细胞MG63增殖能力的影响(2)
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detect the effect of PRP on MG63 cells in different time points. The mRNA level of Col-Ⅰ in MG63 cells cul-tured under different concentration PRP was checked by reverse transcription polymerase chain reaction(RT-PCR).
Results ALP activity experiment demonstrated that the maximum effect was got in 3% PRP group. PRP had a posi-tive effect on the proliferation of MG63 cells but cells also presented disengage phenomena from the glass slides. The PI staining showed that PRP improved fluorescent intensity of cell nucleus ......
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