刘梦云，江洁怡，毕晓黎1，#，李素梅，李养学(1.广州中医药大学研究生院，广州 510405；.广东省中医药工程技术研究院/广东省中医药研究开发重点实验室/中药配方颗粒生产关键技术重点研究室，广州 510095)

    葛花配方颗粒的UPLC指纹图谱研究Δ

    刘梦云1*，江洁怡2，毕晓黎1，2#，李素梅2，李养学2(1.广州中医药大学研究生院，广州 510405；2.广东省中医药工程技术研究院/广东省中医药研究开发重点实验室/中药配方颗粒生产关键技术重点研究室，广州 510095)

    目的：建立葛花配方颗粒的超高效液相色谱(UPLC)指纹图谱。方法：采用UPLC法。色谱柱为Zorbax Eclipse XDB-C18，流动相为乙腈-水(梯度洗脱)，流速为0.5 mL/min，检测波长为264 nm，柱温为25℃，进样体积为1 μL。以鸢尾苷元为参照物，测定10批葛花配方颗粒的UPLC图谱，采用《中药色谱指纹图谱相似度评价系统》(2004A版)进行共有峰指认和相似度评价。结果：10批葛花配方颗粒的UPLC图谱有14个共有峰，相似度均>0.90。经验证，10批样品UPLC图谱与对照指纹图谱具有较好的一致性。结论：该研究所建UPLC指纹图谱可为葛花配方颗粒的鉴别和质量评价提供参考。

    葛花配方颗粒；超高液相色谱法；指纹图谱；鸢尾苷元

    ABSTRACTOBJECTIVE：To establish UPLC fingerprint of Gehua formula granules.METHODS：UPLC method were adopted. The determination was performed on Zorbax Eclipse XDB-C18column with mobile phase consisted of acetonitrile-water at the flow rate of 0.5 mL/min.The detection wavelength was set at 264 nm，column temperature was 25℃，and the sample size was 1 μL. Using tectorigenin as reference substance，UPLC chromatograms of 10 batches of Gehua formula granules were determined.The common peak identification and similarity evaluation were conducted by TCM Chromatogram Fingerprint Similarity Evaluation System(2004 A edition).RESULTS：14 common peaks were identified in UPLC chromatograms of 10 batches of Gehua formula granules and similarities were all higher than 0.90.UPLC chromatograms of 10 batches of samples were in good agreement with control fingerprint.CONCLUSIONS：Established UPLC fingerprint can provide reference for identification and quality evaluation of Gehua formula granules. ......