曾祥燕，唐玉荣，颜萍花，黎 理(广西中医药大学药学院，南宁 530001)

    壮药竹节蓼的质量标准研究Δ

    曾祥燕*，唐玉荣，颜萍花，黎 理#(广西中医药大学药学院，南宁 530001)

    目的：建立壮药竹节蓼的质量标准。方法：采用薄层色谱法(TLC)对药材样品进行定性鉴别；采用高效液相色谱法对药材样品中槲皮苷进行含量测定：色谱柱为Agilent Zorbax SB-C18，流动相为乙腈-0.1%冰醋酸溶液(20∶80，V/V)，流速为1.0 mL/min，检测波长为360 nm，柱温为30℃，进样量为10 μL；参照2015年版《中国药典》测定药材样品水分、灰分和浸出物的含量。结果：竹节蓼药材的TLC图斑点清晰，分离度好。槲皮苷检测质量浓度线性范围为0.023～0.46 mg/mL(r＝0.999 1)；精密度、稳定性、重复性试验的RSD<2.0%；加样回收率为98.01%～103.05%(RSD＝1.71%，n＝6)。药材样品水分、总灰分、酸不溶性灰分、浸出物和槲皮苷含量分别为6.39%～9.78%、2.82%～8.07%、0.02%～0.27%、14.14%～28.45%、0.09%～0.50%。结论：该研究所建标准可用于壮药竹节蓼的质量控制。

    壮药；竹节蓼；槲皮苷；定性；定量；薄层色谱法；高效液相色谱法

    ABSTRACTOBJECTIVE：To establish quality standard for Zhuang medicine Homalocladium platycladum.METHODS：TLC method was used for qualitative identification.HPLC method was used to determine the content of quercetrin in samples：Agilent Zorbax SB-C18column，mobile phase consisted of acetonitrile-0.1%acetic acid(20∶80，V/V)，flow rate of 1.0 mL/min，detection wavelength of 360 nm，column temperature of 30℃，injection volume of 10 μL.The contents of moisture，total ash and extract in samples were determined.referring to Chinese Pharmacopiea(2015 edition).RESULTS：TLC spots of H.platycladum were clear and well-separated without interference from negative control.The linear range of quercetin were 0.023-0.46 mg/mL(r＝0.999 1). RSDs of precision ......