耿婕，那顺朝克图，丛丽华，樊精辉，赵春雨，薛培凤#(.内蒙古医科大学药学院，呼和浩特000；.内蒙古宇航人药业有限责任公司，呼和浩特 057)

    HPLC法同时测定五味沙棘颗粒中甘草苷和甘草酸的含量Δ

    耿婕1*，那顺朝克图1，丛丽华2，樊精辉2，赵春雨1，薛培凤1#(1.内蒙古医科大学药学院，呼和浩特010110；2.内蒙古宇航人药业有限责任公司，呼和浩特 011517)

    目的：建立同时测定五味沙棘颗粒中甘草苷和甘草酸含量的方法。方法：采用高效液相色谱法。色谱柱为Thermo scientific ODS-2 Hypersil，流动相为乙腈-0.5%磷酸溶液(梯度洗脱)，流速为1.0 mL/min，检测波长为237 nm，柱温为25℃，进样量为20 μL。结果：甘草苷和甘草酸检测进样量线性范围分别为48～480 μg(r＝0.999 9)、90～900 μg(r＝0.999 9)；定量限分别为0.55、2.10 μg/mL，检测限分别为0.16、0.62 μg/mL；精密度、稳定性、重复性试验的RSD<2.0%；加样回收率分别为95.08%～97.58%(RSD＝0.93%，n＝6)、95.86%～99.89%(RSD＝1.67%，n＝6)。结论：该方法简单准确、重复性好，适用于五味沙棘颗粒中甘草苷和甘草酸含量的同时测定。

    高效液相色谱法；五味沙棘颗粒；甘草苷；甘草酸；含量测定

    ABSTRACTOBJECTIVE：To establish a method for simultaneous determination of liquiritin and glycyrrhizic acid in Wuwei shaji granules.METHODS：HPLC method was adopted.The determination was performed on Thermo scientific ODS-2 Hypersil column with mobile phase consisted of acetonitrile-0.5%phosphoric acid(gradient elution)with flow rate of 1.0 mL/min.The detection wavelength was set at 237 nm，and column temperature was 25 ℃.The sample size was 20 μL.RESULTS：The linear ranges of liquiritin and glycyrrhizic acid were 48-480 μg(r＝0.999 9)and 90-900 μg(r＝0.999 9) ......