严丹，刘玉杰，胡美变，肖禾，李永川，吴纯洁，3#(.成都中医药大学药学院，成都637；.成都岷江源药业股份有限公司，成都 6000；3.国家中医药管理局中药炮制技术重点研究室，成都 673)

    炮山甲的HPLC指纹图谱研究Δ

    严丹1*，刘玉杰1，胡美变1，肖禾2，李永川2，吴纯洁1，3#(1.成都中医药大学药学院，成都611137；2.成都岷江源药业股份有限公司，成都 611000；3.国家中医药管理局中药炮制技术重点研究室，成都 611731)

    目的：建立炮山甲的高效液相色谱(HPLC)指纹图谱。方法：采用HPLC法。色谱柱为Capcell Pak MgⅡS5 C18，流动相为乙腈-0.1%磷酸溶液(梯度洗脱)，流速为0.8 mL/min，检测波长为275 nm，柱温为30℃，进样量为10 μL。以酪氨酸为参照，测定11批药材的HPLC图谱，采用《中药色谱指纹图谱相似度评价系统》(2004A版)进行共有峰指认和相似度评价。结果：11批炮山甲药材的HPLC图谱有23个共有峰，相似度均>0.90。经验证，11批药材样品HPLC图谱与对照指纹图谱具有较好的一致性。结论：该研究所建HPLC指纹图谱可为炮山甲的鉴别和质量评价提供参考。

    炮山甲；高效液相色谱法；指纹图谱；质量评价

    ABSTRACTOBJECTIVE：To establish HPLC fingerprint of stir-baked Manis pentadactyla.METHODS：HPLC method was conducted.The determination was performed on Capcell Pak MgⅡS5 C18column with mobile phase consisted of acetonitrile-0.1%phosphoric acid solution(gradient elution)at the flow rate of 0.8 mL/min.The detection wavelength was set at 275 nm，and column temperature was 30 ℃.The sample size was 10 μL.Using oxyphenylaminopropionic acid as reference，HPLC chromatograms of 11 batches of medicinal materials were determined.TCM Chromatogram Fingerprint Similarity Evaluation System(2004 A edition)was used for common peak identification and similarity evaluation.RESULTS：There were 23 common peaks in 11 batches of stir-baked M.pentadactyla ......