黄秋洁，叶勇，王捷，王志萍，韦炜，李丹，朱滴新(1.广西中医药大学药学院，南宁50001；2.广西高校中药制剂共性技术研发重点实验室，南宁 50001；.广西医科大学药学院，南宁 50021；.广西医科大学制药厂，南宁 50021)

    广西地不容药材的HPLC指纹图谱研究Δ

    黄秋洁1，2*，叶勇3#，王捷4，王志萍1，2，韦炜3，李丹3，朱滴新3(1.广西中医药大学药学院，南宁530001；2.广西高校中药制剂共性技术研发重点实验室，南宁 530001；3.广西医科大学药学院，南宁 530021；4.广西医科大学制药厂，南宁 530021)

    目的：建立广西地不容药材的高效液相色谱(HPLC)指纹图谱。方法：采用HPLC法。色谱柱为Hypersil ODS2，流动相为乙腈-0.5%磷酸溶液(梯度洗脱)，流速为1.0 mL/min，检测波长为282 nm，柱温为30℃，进样量为10 μL。以延胡索乙素为参照，测定10批药材样品的HPLC图谱，采用《中药色谱指纹图谱相似度评价系统》(2012版)进行共有峰指认和相似度评价。结果：10批广西地不容的HPLC图谱有17个共有峰，10批药材样品中有8个产地药材指纹图谱与对照图谱比较相似度>0.900，2个产地药材相似度<0.900。结论：该研究所建HPLC指纹图谱可为广西地不容的鉴别和质量评价提供参考；广西区内多数产地的地不容药材所含生物碱类成分相似，少数存在差异。

    广西地不容；高效液相色谱法；指纹图谱；相似度

    ABSTRACTOBJECTIVE：To establish HPLC fingerprints of Stephania kwangsiensis.METHODS：HPLC method was adopted.The determination was performed on Hypersil ODS2 with mobile phase consisted of acetonitrile-0.5%phosphoric acid(gradient elution)at the flow rate of 1.0 mL/min.The detection wavelength was set at 282 nm，and column temperature was 30℃.The sample size was 10 μL.Using tetrahydropalmatine as reference，HPLC fingerprints of 10 batches of medicinal materials were determined.Common peak identification and similarity evaluation were conducted by using Similarity Evaluation System of TCM Chromatographic Fingerprint(2012 edition).RESULTS：A total of 17 common peaks were identified in HPLC fingerprints of 10 batches of S.kwangsiensis.Among 10 batches of samples ......