杨家强 车万莉 彭红艳 王维

    中图分类号 R283 文献标志码 A 文章編号 1001-0408(2018)13-1802-04

    DOI 10.6039/j.issn.1001-0408.2018.13.18

    摘 要 目的：优化荜茇总生物碱的提取工艺，为荜茇的深入开发和综合利用提供参考。方法：以总生物碱(指标性成分为胡椒碱)的提取率为考察指标，药材粉碎度、液料比、提取时间、提取次数为考察因素，采用单因素试验和Box-Behnken响应面法优化荜茇总生物碱的提取工艺，并进行3次(荜茇用量分别为1.00、10.00、100.00 g)验证试验。结果：最优提取工艺为粉碎度90目，加入32倍量的无水乙醇提取3次，每次提取170 min。最优工艺条件下，在荜茇用量为1.00、10.00、100.00 g时荜茇总生物碱的提取率分别为5.015、5.028、5.041 mg/g，与预测值5.025 mg/g的相对误差分别为0.20%、0.06%、0.26%，差异较小。结论：本研究优选的荜茇总生物碱的提取工艺合理、稳定、可行。

    关键词 荜茇；总生物碱；Box-Behnken响应面法；提取率；提取工艺

    ABSTRACT OBJECTIVE： To optimize the extraction technology of total alkaloids from Piper longum， and to provide reference for deep development and comprehensive utilization of P. longum. METHODS： With the extraction rates of total alkaloids (the target component for piperine) as reference index， using comminution degree of medicinal materials， liquid-solid ratio， extraction time and extraction times as factors， single factor test and Box-Behnken response surface methodology were adopted to optimize the extraction technology of total alkaloids from P. longum. The validation tests were conducted for 3 times (the amounts of P. longum were 1.00 ......