赤苍藤茎叶水提物抗痛风作用的实验研究(1)
摘 要 目的：研究赤苍藤茎叶水提物(ASLE)的抗痛风作用。方法：取小鼠随机分为正常组、模型组、别嘌醇组(阳性对照，5 mg/kg)和ASLE低、中、高剂量组(1 300、2 600、5 200 mg/kg，按生药量计;下同)，每组10只。除正常组外，其余各组小鼠均灌胃氧嗪酸钾以复制高尿酸血症模型;造模1 h后，正常组和模型组小鼠均灌胃等体积生理盐水，各给药组小鼠灌胃相应药物，每日1次，连续7 d。末次给药后1 h，采用酶比色法检测各组小鼠血尿酸(SUA)、血肌酐(Scr)水平。另取小鼠随机分为正常组、模型组、吲哚美辛组(阳性对照，7.5 mg/kg)和ASLE低、中、高剂量组，每组10只。正常组和模型组小鼠均灌胃等体积生理盐水，各给药组小鼠灌胃相应药物，每日1次，连续7 d。末次给药后，除正常组外，其余各组小鼠均于足趾部注射微晶尿酸钠以复制痛风性关节炎模型。分别于造模前及造模后1、2、4、6、8 h时采用缚线法测量各组小鼠致炎肢足趾同一部位周径，并计算足趾肿胀度;使用动物血液分析仪检测其白细胞(WBC)、中性粒细胞(NEU)和淋巴细胞(LYM)计数;采用酶比色法检测血清SUA、Scr水平;采用Griess法检测足趾组织中一氧化氮(NO)的含量。结果：高尿酸血症模型实验结果显示，模型组小鼠血清SUA、Scr水平均较正常组显著升高(P<0.01);与模型组比较，各给药组小鼠上述指标均显著降低(P<0.05或P<0.01)。痛风性关节炎模型实验结果显示，模型组小鼠血清SUA水平，足趾肿胀度(2～8 h)，WBC、NEU、LYM计数以及NO含量均较正常组显著升高(P<0.05或P<0.01);与模型组比较，不同给药组小鼠SUA、Scr水平(ASLE各剂量组)，足趾肿胀度[吲哚美辛组和ASLE高劑量组(2～8 h)、ASLE低剂量组(2、6 h)、ASLE中剂量组(6 h)]，WBC和NEU计数(各给药组)，LYM计数(吲哚美辛组)以及NO含量(除ASLE低剂量组外的各给药组)均显著降低(P<0.05或P<0.01)。结论：ASLE的抗痛风作用可能与促进尿酸代谢、抗炎以及保护或改善肾功能等有关。
关键词 赤苍藤;茎叶;水提物;痛风;高尿酸血症;痛风性关节炎;小鼠
ABSTRACT OBJECTIVE： To study the anti-gout effect of aqueous extract from the stems and leaves of Erythropalum scandens (ASLE). METHODS： The mice were randomly divided into normal group， model group， allopurinol group (positive control， 5 mg/kg)， ASLE low-dose， medium-dose and high-dose groups (1 300， 2 600， 5 200 mg/kg， by raw material; similarity hereinafter)， with 10 mice in each group. Except for normal group， other groups were given potassium oxonate intragastrically to induce hyperuricemia model. One hour after modeling， normal group and model group were given constant volume of normal saline intragastrically; administration group was given relevant medicine intragastrically， once a day， for consecutive 7 d. One hour after last administration， the levels of serum uric acid (SUA) and serum creatinine (Scr) were detected by colorimetry assay. Another mice were randomly divided into normal group， model group， indomethacin group (positive control， 7.5 mg/kg)， ASLE low-dose， medium-dose and high-dose groups， with 10 mice in each group. Normal group and model group were given constant volume of normal saline intragastrically; administration group was given relevant medicine intragastrically， once a day， for consecutive 7 d. After last administration， except for normal group， the mice were given sodium microcrystalline urate via toes to induce gouty arthritis model. Before and 1， 2， 4， 6， 8 h after modeling， the circumference of the same part of the inflamed limbs and toes of mice in each group was measured by wire binding method， and the degree of toe swelling was calculated. The number of white blood cell (WBC)， neutrophil (NEU) and lymphocyte (LYM) were detected by animal hematology analyzer. The levels of SUA and Scr were measured by colorimetry assay. The content of NO in toe tissue was determined by Griess method. RESULTS： The experimental results of hyperuricemia model showed that the levels of SUA and Scr in mice were significantly higher in model group than those in normal group (P<0.01). Compared with model group， above indexes of mice were decreased significantly in administration group (P<0.05 or P<0.01). The experimental results of gouty arthritis model showed that the level of SUA， the degree of toe swelling (2-8 h)， the number of WBC， NEU and LYM， NO content in model group were increased significantly， compared with normal group (P<0.05 or P<0.01). Compared with model group， the levels of SUA and Scr (ASLE groups)， the degree of toe swelling [indomethacin group， ASLE high-dose group (2-8 h)， ASLE low-dose group (2， 6 h)， ASLE medium-dose group (6 h)]， the number of WBC and NEU (administration groups)， the number of LYM (indomethacin group) and NO content (administration groups except for ASLE low-dose group) were decreased significantly in administration groups (P<0.05 or P<0.01). CONCLUSIONS： The anti-gout effect of ASLE may be associated with promoting uric acid metabolism， anti-inflammatory and improving renal function., 百拇医药(许崇摇 韦贵云 朱丹 王璐琪 周秋妹 蒋伟哲)
关键词 赤苍藤;茎叶;水提物;痛风;高尿酸血症;痛风性关节炎;小鼠
ABSTRACT OBJECTIVE： To study the anti-gout effect of aqueous extract from the stems and leaves of Erythropalum scandens (ASLE). METHODS： The mice were randomly divided into normal group， model group， allopurinol group (positive control， 5 mg/kg)， ASLE low-dose， medium-dose and high-dose groups (1 300， 2 600， 5 200 mg/kg， by raw material; similarity hereinafter)， with 10 mice in each group. Except for normal group， other groups were given potassium oxonate intragastrically to induce hyperuricemia model. One hour after modeling， normal group and model group were given constant volume of normal saline intragastrically; administration group was given relevant medicine intragastrically， once a day， for consecutive 7 d. One hour after last administration， the levels of serum uric acid (SUA) and serum creatinine (Scr) were detected by colorimetry assay. Another mice were randomly divided into normal group， model group， indomethacin group (positive control， 7.5 mg/kg)， ASLE low-dose， medium-dose and high-dose groups， with 10 mice in each group. Normal group and model group were given constant volume of normal saline intragastrically; administration group was given relevant medicine intragastrically， once a day， for consecutive 7 d. After last administration， except for normal group， the mice were given sodium microcrystalline urate via toes to induce gouty arthritis model. Before and 1， 2， 4， 6， 8 h after modeling， the circumference of the same part of the inflamed limbs and toes of mice in each group was measured by wire binding method， and the degree of toe swelling was calculated. The number of white blood cell (WBC)， neutrophil (NEU) and lymphocyte (LYM) were detected by animal hematology analyzer. The levels of SUA and Scr were measured by colorimetry assay. The content of NO in toe tissue was determined by Griess method. RESULTS： The experimental results of hyperuricemia model showed that the levels of SUA and Scr in mice were significantly higher in model group than those in normal group (P<0.01). Compared with model group， above indexes of mice were decreased significantly in administration group (P<0.05 or P<0.01). The experimental results of gouty arthritis model showed that the level of SUA， the degree of toe swelling (2-8 h)， the number of WBC， NEU and LYM， NO content in model group were increased significantly， compared with normal group (P<0.05 or P<0.01). Compared with model group， the levels of SUA and Scr (ASLE groups)， the degree of toe swelling [indomethacin group， ASLE high-dose group (2-8 h)， ASLE low-dose group (2， 6 h)， ASLE medium-dose group (6 h)]， the number of WBC and NEU (administration groups)， the number of LYM (indomethacin group) and NO content (administration groups except for ASLE low-dose group) were decreased significantly in administration groups (P<0.05 or P<0.01). CONCLUSIONS： The anti-gout effect of ASLE may be associated with promoting uric acid metabolism， anti-inflammatory and improving renal function., 百拇医药(许崇摇 韦贵云 朱丹 王璐琪 周秋妹 蒋伟哲)
参见：首页 > 医疗版 > 疾病专题 > 内分泌科 > 痛风