中图分类号 R93.4；R97 文献标志码 A 文章编号 00-0408(05)-37-06

    DOI 0.6039/j.issn.00-0408.05..05

    ABSTRACT OBJECTIVE To screen the primary processing methods of Ligusticum sinense slice based on differential metabolites，and provide theoretical basis for the scientific processing of L . sinense. METHODS Using 13 groups of L . sinense slice processed by fresh-cutting or traditional methods as samples，UHPLC-QE-MS was employed for metabolite identification. Multivariate statistical analysis was applied to screen differential metabolites among the 13 sample groups，analyzing the effects of washing，soaking，drying methods，and drying cycles on both the relative expressions of differential metabolites and the contents of carboxylic acids and their derivatives in the samples(to reflect the total amino acid content). RESULTS Principal component analysis and partial least squares-discriminant analysis both showed significant intergroup differences among the 13 sample groups. A total of 688 differential metabolites were screened from the 13 sample groups，with carboxylic acids and their derivatives showing the highest proportion. The relative expression levels of phosphatidylcholine significantly increased after washing treatment ......