中图分类号 R917；R944 文献标志码 A 文章编号 1001-0408(2025)11-1335-05

    ABSTRACT OBJECTIVE To investigate the absorption characteristics and tissue distribution of silybin (Sy) nanocrystals prepared by two methods in different intestinal segments of rats. METHODS Sy nanocrystals(i. e. Sy-NS-G and Sy-NS-F)with comparable particle sizes were prepared using high-pressure homogenization and anti-solvent precipitation methods，respectively. Rats were randomly divided into three groups：Sy raw drug group，Sy-NS-G group，and Sy-NS-F group. Each group was further divided into three subgroups with low，medium，and high(60，120， 180μg/mL )mass concentrations(calculated based on Sy)， with 3 rats in each subgroup. The absorption rate constant (K_a) and apparent absorption coefficient( (P_app) )of Sy raw drug，Sy-NS-G and Sy-NS-F in different intestinal segments were investigated by using the in vivo one-way intestinal perfusion experiment. Additionally，the rats were divided into three groups： Sy raw drug group，Sy-NS-G group，and Sy-NS-F group，with 20 rats in each group. Rats in each group were administered a single intragastric dose of 50mg/kg (calculated based on Sy). They were sacrificed at 0.3 ......