中图分类号 R917；R84.1 文献标志码 A 文章编号 1001-0408(05)11-1-06

    DOI 10.609/j.issn.1001-0408.05.11.06

    ABSTRACT OBJECTIVE To establish a quantitative analysis of multi-components by single-marker (QAMS) method for simultaneous determination of six flavonoids and two phenolic acids in Perilla frutescens leaves using scutellarin and rosmarinic acid as internal reference substances，and apply this method to determine the contents of eight components in 20 batches of P. . frutescens leaves samples from different regions. METHODS Scutellarin served as the internal reference to calculate relative correction factors (RCFs) for scutellarin-7- O -diglucuronide，luteolin-7- O -diglucuronide，apigenin-7-O-diglucuronide，luteolin-7-Oβ -D-glucuronide and apigenin-7-O-glucuronide. Rosmarinic acid was employed as the internal reference to determine the RCF for caffeic acid. The contents of the above flavonoids and phenolic acids were calculated with QAMS，and compared with the results of external standard method. RESULTS The eight analytes demonstrated excellent linearity within their respective concentration ranges ( r?0.999 0 ). The mean recovery rates for spiked samples ranged from 95.60% to 102.15% ......