中图分类号 R965；R285.5 文献标志码 A 文章编号 00-0408(2025)-52-06

    DOI 0.609/j.issn.00-0408.2025..

    ABSTRACT OBJECTIVE To study the effects and mechanism of Portulaca oleracea cream on skin pruritus and barrier function in allergic contact dermatitis (ACD) mice. METHODS Low-concentration and high-concentration P. oleracea creams were prepared，with the P. oleracea extract solution( (1g/mL ，calculated by crude drug)concentrations of 10% and 20% . Sixty BALB/c mice were randomly allocated into blank group，model group，Mometasone furoate cream group(positive control)，blank matrix cream group，P. oleracea low-concentration and high-concentration cream groups. Except for blank group，ACD model was induced in each group using 2，4-dinitrochlorobenzene solution. The blank group and model groups received normal saline，while the remaining groups were treated with their respective creams，once a day，at a dose of approximately 0.5g per application， continuously for 14 days. At 24h post-final administration，skin lesions of mice were observed and scored；pathological changes of skin tissue were observed；serum levels of immunoglobulin E (IgE) and tumor necrosis factor- α (TNF- α ) were quantified. mRNA expression of MAS-related G protein-coupled receptors (including MrgprA3 ......