中图分类号R965；R285 文献标志码A 文章编号 1001-0408(2025)14-1722-08

    DOI 10.6039/j.issn.1001-0408.2025.14.06

    ABSTRACTOBJECTIVEToexplore the mechanismof Shenqi guben formula(SQGB)in improving cancer-related fatigue (CRF)based on network pharmacologyand celular experiments.METHODS Activecomponentsof SQGB and CRF-related targetswereidentifiedonthebasisofdatabasessuchastheTraditionalChineseMedicineSystemsPharmacologyplatform.Anin vitro CRFcellmodel was establishedby inducing A549celswith interleukin-17(IL-17).Cells were treated with low( 1.0mg/mL )orhigh (1.5mg/mL )concentrations of SQGB.The effectson cellviability，migration，apoptosis，inflammatoryfactors， mRNA expression，apoptosis-related proteins and key proteins of IL-17 signaling pathway were evaluated using scratch assay， flowcytometry，ELISA，real-time fluorescent quantitative PCR and Western blot analysis.RESULTS SQGB contained 84 active components acting on 209 potential CRF targets. Among these，quercetin，kaempferol，and luteolin were identified as thecore components of the compound.Core targets included tumorproteip53，AKTserie/threoninekinase1 ......