叶酸修饰的克班宁纳米粒联合超声辐照对M109细胞的体内外靶向作用及抗肿瘤机制
中图分类号R944 文献标志码A 文章编号 1001-0408(2025)14-1730-07
DOI 10.6039/j.issn.1001-0408.2025.14.07
ABSTRACTOBJECTIVETo investigatethe targeting efectoffolicacid-modified crebanine nanoparticles(FA-Cre@PEGPLGANPs，hereinafterreferredtoas“NPs”)combinedwithultrasoundirrdiationonM109cellsinvitroandinvivoafter adminstration，andexplore theant-tumormechanism.METHODSCCK-8assywasusedtodetectthe inhibitoryefectof NPs combinedwithultrasoundiradiationontheproliferationofM109cells，andthebestultrasoundtimewasseleced.Usinghuman lungcancerA549celsasacontrol，thetargetingofNPscombinedwithultrasoundiradiationtoM109celswasevaluatedbyfree folicacid blocking assay and cell uptake assay.The effectsof NPs combined with ultrasound irradiation on the migration， invasion，apoptosis，cell cycle and reactive oxygen species (ROS)levelsofM1o9 cellswere detectedbycell scratch test， Transwell chamber test and flow cytometry at 1hafter administration； the changesof mitochondrial membrane potential (MMP)were observed by fluorescence inverted microscope.A mouse subcutaneoustumormodelofMlo9 cells wasconstructed ......
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