丹皮酚调控NF-κB/HIF-1α信号通路抑制膀胱癌T24细胞迁移的作用及机制
中图分类号R965 文献标志码A 文章编号 1001-0408(2025)15-1871-05
DOI 10.6039/j.issn.1001-0408.2025.15.09
Effect and mechanism of paeonol in regulating NF- κκκκB/HIF -lαsignaling pathway to inhibit the migration ofbladder cancer T24 cells
AI Xinyao，CHEN Wenjia，CHEN Xi，WANG Yingzheng，WANG Yinghao，HUANG Meixia(Schoolof Pharmacy，Fujian University of Traditional Chinese Medicine，Fuzhou 350122，China)
ABSTRACTOBJECTIVEToinvestigatetheroleand mechanismof paeonolininhibitingthemigrationofbladdercancerT24 cells by regulating nuclear factor κB (NF- κB )/hypoxia-inducible factor- 1α (HIF- ?l∝ )-mediated aerobic glycolysis.METHODS T24 cells were divided into control group，cisplatin group (positive control， 3.001μg/mL )，and paeonol low-，medium- and high-dose groups(100，200， 400μg/mL )，respectively. After 24h of administration intervention，the effect of paeonol on the migration abilityofT24cellswasdetected(expressedbytecellscratchwoundhealingrate).Theefectofpaeonolonthemitochondrial membranepotentialof T24celswasdetected(expressedbytheratioofred/green fluorescenceintensity).Celularadenosine triphosphate(ATP)levelsand lactate content in T24celswere measured.The levelsof NF -κB/HIF-lα signaling pathway ......
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