莪术醇抑制人肝癌细胞HepG2增殖的机制(4)
2. School of Pharmacy， Guilin Medical University， Guilin 541004， China)
[Abstract] Objective： To investigate the anti-proliferative effects of curcumol，an herbal extract from curcuma，in human hepatocarcinoma HepG2 cells，and its possible molecular mechanism. Method： The effects of curcumol on human hepatocarcinoma cells were assessed in vitro. Proliferation of HepG2 cells treated with various concentration(2.5-10 mg·L-1)of curcumol was determined using the MTT assay and the distribution of cell cycle of HepG2 cells was analyzed using the FCM technique. Expression of 14 cell cycle regulation-related genes were assessed by TaqMan real-time polymerase chain reaction (RT-PCR) method and Western blot. Result： Curcumol significantly inhibited the proliferation of HepG2 cells and induced G1 phase arrest in a dose- and time-dependent manner. The mRNA levels of pRB1，cyclin D1，CDK2，CDK8 and p27KIP1 were elevated，while cyclin A1 decreased，in both of the low (25 mg·L-1) and the high dose (100 mg·L-1) treatment of curcumol. There were no significant changes in the expression of either cyclin E1 or CDK4. The expression of p53 and its target genes p21WAF1 and Wip1 protein were increased. Conclusion： Curcumol can inhibit the proliferation of HepG2 cells in vitro and induce G 1 arrest of cell cycle through mechanisms activating p53 and pRB pathways that involve genes of cyclin A1，CDK2，CDK8，p21WAF1 and p27KIP1.
[Key words] curcumol； hepatocarcinoma； tumor cell proliferation； cell cycle arrest
doi：10.4268/cjcmm20131132
[责任编辑 张宁宁], 百拇医药(黄岚珍,王娟,卢菲婷,杨飞城,陈旭,洪雪,蒋晓山)
[Abstract] Objective： To investigate the anti-proliferative effects of curcumol，an herbal extract from curcuma，in human hepatocarcinoma HepG2 cells，and its possible molecular mechanism. Method： The effects of curcumol on human hepatocarcinoma cells were assessed in vitro. Proliferation of HepG2 cells treated with various concentration(2.5-10 mg·L-1)of curcumol was determined using the MTT assay and the distribution of cell cycle of HepG2 cells was analyzed using the FCM technique. Expression of 14 cell cycle regulation-related genes were assessed by TaqMan real-time polymerase chain reaction (RT-PCR) method and Western blot. Result： Curcumol significantly inhibited the proliferation of HepG2 cells and induced G1 phase arrest in a dose- and time-dependent manner. The mRNA levels of pRB1，cyclin D1，CDK2，CDK8 and p27KIP1 were elevated，while cyclin A1 decreased，in both of the low (25 mg·L-1) and the high dose (100 mg·L-1) treatment of curcumol. There were no significant changes in the expression of either cyclin E1 or CDK4. The expression of p53 and its target genes p21WAF1 and Wip1 protein were increased. Conclusion： Curcumol can inhibit the proliferation of HepG2 cells in vitro and induce G 1 arrest of cell cycle through mechanisms activating p53 and pRB pathways that involve genes of cyclin A1，CDK2，CDK8，p21WAF1 and p27KIP1.
[Key words] curcumol； hepatocarcinoma； tumor cell proliferation； cell cycle arrest
doi：10.4268/cjcmm20131132
[责任编辑 张宁宁], 百拇医药(黄岚珍,王娟,卢菲婷,杨飞城,陈旭,洪雪,蒋晓山)