油樟叶挥发油β—环糊精包合物的制备工艺研究及稳定性考察(3)
Study on preparation process and stability of β-cyclodextrin inclusion compound in volatile oil of Cinnamomum longepaniculatum leaves
LIU Yu1，3， YIN Zhong-qiong1*， WEI Qin2*， JIA Ren-yong1， FAN Jing1， ZHOU Li-jun2， DU Yong-hua2
(1. College of Veterinary Medicine， Sichuan Agricultural University， Ya′an 625014， China；
2. Key Laboratory of Fermentation Resource and Application of Higher Education Institutions in Sichuan， Yibin 644000， China；
3. Luxian County Bureau of Animal Husbandry of Sichuan Province， Luzhou 646100， China)
[Abstract] To study the optimum preparation process and stability of β-cyclodextrin inclusion compound in volatile oil of Cinnamomum longepaniculatum leaves. The saturated aqueous solution method was adopted to prepare inclusion compounds for an orthogonal test. The inclusion compound productivity and the inclusion rate were taken as indexes for screening the inclusion processes. The inclusion effect was evaluated with the infrared spectrophotometry and TLC， and the stability under conditions of high temperature， high humidity and strong light was detected. Under optimum preparation conditions for inclusion， the ratio between volatile oil and β-cyclodextrin was 1∶8 (mL∶g)， that between β-cyclodextrin and water was 1∶15， the inclusion temperature was 40 ℃， and the inclusion time was 3 h. The results of spectrophotometry and TLC showed that the optimum conditions can generate β-cyclodextrin inclusion compound in volatile oil of C. longepaniculatum leaves with certain light resistance， thermo-stability and hygro-stability. Therefore the optimum inclusion process features simple operation and stable inclusion compounds.
[Key words] volatile oil of Cinnamomum longepaniculatum leaf； β-cyclodextrin； characterization； stability
doi：10.4268/cjcmm20131314
[责任编辑 曹阳阳], http://www.100md.com(刘宇，殷中琼，魏琴，贾仁勇，范静，周黎军，杜永华)
LIU Yu1，3， YIN Zhong-qiong1*， WEI Qin2*， JIA Ren-yong1， FAN Jing1， ZHOU Li-jun2， DU Yong-hua2
(1. College of Veterinary Medicine， Sichuan Agricultural University， Ya′an 625014， China；
2. Key Laboratory of Fermentation Resource and Application of Higher Education Institutions in Sichuan， Yibin 644000， China；
3. Luxian County Bureau of Animal Husbandry of Sichuan Province， Luzhou 646100， China)
[Abstract] To study the optimum preparation process and stability of β-cyclodextrin inclusion compound in volatile oil of Cinnamomum longepaniculatum leaves. The saturated aqueous solution method was adopted to prepare inclusion compounds for an orthogonal test. The inclusion compound productivity and the inclusion rate were taken as indexes for screening the inclusion processes. The inclusion effect was evaluated with the infrared spectrophotometry and TLC， and the stability under conditions of high temperature， high humidity and strong light was detected. Under optimum preparation conditions for inclusion， the ratio between volatile oil and β-cyclodextrin was 1∶8 (mL∶g)， that between β-cyclodextrin and water was 1∶15， the inclusion temperature was 40 ℃， and the inclusion time was 3 h. The results of spectrophotometry and TLC showed that the optimum conditions can generate β-cyclodextrin inclusion compound in volatile oil of C. longepaniculatum leaves with certain light resistance， thermo-stability and hygro-stability. Therefore the optimum inclusion process features simple operation and stable inclusion compounds.
[Key words] volatile oil of Cinnamomum longepaniculatum leaf； β-cyclodextrin； characterization； stability
doi：10.4268/cjcmm20131314
[责任编辑 曹阳阳], http://www.100md.com(刘宇，殷中琼，魏琴，贾仁勇，范静，周黎军，杜永华)