银杏外种皮多糖的单糖组成分析(4)
[13]Honda S,Akao E,Suzuki S,et al.High-performance liquid chroma-tography of reducing carbohydrates as strongly ultraviolet-absorbing and electrochemically sensitive 1-phenyl-3-methyl-5-pyrazolonederivatives[J].Anal Biochem,1989,180(2):351.
[14]吕永磊,卜海博,杨蕾,等. 乌头母根子根须根多糖的比较[J].中国中药杂志,2011,36(9):1154.
[15]Dai J,Wu Y, Chen S W,et al.Sugar compositional determination of polysaccharides from Dunaliella salina by modified RP-HPLC method of precolumn derivatization with 1-phenyl-3-methyl-5-pyrazolone[J].Carbohydr Polym,2010,82:629.
[16]宋根萍,许爱华,陈华圣,等.银杏外种皮多糖的成分分析[J].中药材,1997,20(9):461.
Analysis of monosaccharide compositions in polysaccharides
from exopleura of Ginkgo biloba
MAO Lei-lei, CHEN Ying, HU Bi-yuan, XU Ai-hua*
(Medical College of Yangzhou University, Yangzhou 225001, China)
[Abstract]A high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) has been established for determination of 6 kinds of monosaccharides simultaneously. A special Agilent HC-C18 column (4.6 mm×250 mm,5 μm), optimized for the separation of PMP derivatives, was used at ambient temperature of 40 ℃. The PMP derivatives elution was performed with a mixture of 0.1 mol·L-1 phosphate buffer (pH 6.8) and acetonitrile in a ratio of 84∶16 at a flow rate of 1 mL·min-1, and UV absorbance of the effluent was monitored at 245 nm. The results showed that the polysaccharides from exopleura of Ginkgo biloba were acidic heteropolysaccharides mainly containing mannose,rhamnose, D-galacturonic acid,glucose, galactose, arabinose, with the molar ratio of 0.032∶0.14∶0.296∶0.403∶0.106∶0.046.
[Key words]Ginkgo biloba; polysaccharides; monosaccharide; PMP; HPLC
doi:10.4268/cjcmm20140220
[责任编辑孔晶晶], 百拇医药(茅蕾蕾 陈颖 胡碧原 许爱华)
[14]吕永磊,卜海博,杨蕾,等. 乌头母根子根须根多糖的比较[J].中国中药杂志,2011,36(9):1154.
[15]Dai J,Wu Y, Chen S W,et al.Sugar compositional determination of polysaccharides from Dunaliella salina by modified RP-HPLC method of precolumn derivatization with 1-phenyl-3-methyl-5-pyrazolone[J].Carbohydr Polym,2010,82:629.
[16]宋根萍,许爱华,陈华圣,等.银杏外种皮多糖的成分分析[J].中药材,1997,20(9):461.
Analysis of monosaccharide compositions in polysaccharides
from exopleura of Ginkgo biloba
MAO Lei-lei, CHEN Ying, HU Bi-yuan, XU Ai-hua*
(Medical College of Yangzhou University, Yangzhou 225001, China)
[Abstract]A high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) has been established for determination of 6 kinds of monosaccharides simultaneously. A special Agilent HC-C18 column (4.6 mm×250 mm,5 μm), optimized for the separation of PMP derivatives, was used at ambient temperature of 40 ℃. The PMP derivatives elution was performed with a mixture of 0.1 mol·L-1 phosphate buffer (pH 6.8) and acetonitrile in a ratio of 84∶16 at a flow rate of 1 mL·min-1, and UV absorbance of the effluent was monitored at 245 nm. The results showed that the polysaccharides from exopleura of Ginkgo biloba were acidic heteropolysaccharides mainly containing mannose,rhamnose, D-galacturonic acid,glucose, galactose, arabinose, with the molar ratio of 0.032∶0.14∶0.296∶0.403∶0.106∶0.046.
[Key words]Ginkgo biloba; polysaccharides; monosaccharide; PMP; HPLC
doi:10.4268/cjcmm20140220
[责任编辑孔晶晶], 百拇医药(茅蕾蕾 陈颖 胡碧原 许爱华)