用于中药复方物质基础研究的Caco—2细胞胶原包被6孔转运板体系的构建(4)
[21] 鄢良春， 刘青春， 赵军宁，等. 小柴胡汤在Caco-2细胞模型的吸收特性和转运机制研究[J]. 中国中药杂志， 2011， 36(8)：1087.
Establishment of Caco-2 cell monolayer model with collagen coating 6-well
plates for study of traditional Chinese medicine prescription
YANG Yan-fang， WU Ni， YANG Xiu-wei*
(Department of Natural Medicines， State Key Laboratory of Natural and Biomimetic Drugs，School of Pharmaceutical Sciences， Peking University， Beijing 100191， China)
[Abstract] Caco-2 cell monolayer model is widely utilized in drug absorption study and 12-well transwellTM plates were commonly used to study the absorption of different kinds of natural products. To establish a stable method for the study of traditional Chinese medicine prescription， 6-well plates were chosen because of the larger well volumes than 12-well plates. To study the impacts of collagen kinds， coating density as well as coating time on the cell culture， the transepithelial electrical resistance of Caco-2 cell monolayers grown on different collagen coating transwells was determined， and the permeations of propranolol and atenolol as standard markers were detected with HPLC. The results showed that the kinds of collagen， the different coating densities and coating time of rat tail collagen had no significant influences on the Caco-2 cell monolayer integrality and absorption capacity. 6-well plates coated with 2 μg·cm-2 rat tail collagen for 1 hour were enough reliable and suitable for the study of traditional Chinese medicine prescription in vitro.
[Key words] Caco-2 cell monolayer； 6-well plates； collagen； transepithelium electrical resistance； apparent permeability coefficients
doi：10.4268/cjcmm20140330
[责任编辑 陈玲], 百拇医药(杨雁芳 吴妮 杨秀伟)
Establishment of Caco-2 cell monolayer model with collagen coating 6-well
plates for study of traditional Chinese medicine prescription
YANG Yan-fang， WU Ni， YANG Xiu-wei*
(Department of Natural Medicines， State Key Laboratory of Natural and Biomimetic Drugs，School of Pharmaceutical Sciences， Peking University， Beijing 100191， China)
[Abstract] Caco-2 cell monolayer model is widely utilized in drug absorption study and 12-well transwellTM plates were commonly used to study the absorption of different kinds of natural products. To establish a stable method for the study of traditional Chinese medicine prescription， 6-well plates were chosen because of the larger well volumes than 12-well plates. To study the impacts of collagen kinds， coating density as well as coating time on the cell culture， the transepithelial electrical resistance of Caco-2 cell monolayers grown on different collagen coating transwells was determined， and the permeations of propranolol and atenolol as standard markers were detected with HPLC. The results showed that the kinds of collagen， the different coating densities and coating time of rat tail collagen had no significant influences on the Caco-2 cell monolayer integrality and absorption capacity. 6-well plates coated with 2 μg·cm-2 rat tail collagen for 1 hour were enough reliable and suitable for the study of traditional Chinese medicine prescription in vitro.
[Key words] Caco-2 cell monolayer； 6-well plates； collagen； transepithelium electrical resistance； apparent permeability coefficients
doi：10.4268/cjcmm20140330
[责任编辑 陈玲], 百拇医药(杨雁芳 吴妮 杨秀伟)