缺血性中风病患者体质、证候与MMP9，IL6，MTHFR基因多态性的相关性研究(1)
[摘要]通過研究缺血性中风病患者证候、体质与MMP9，IL6，MTHFR基因多态性之间相互关系，对缺血性中风病患者从体质、证候与MMP9，IL6，MTHFR基因多态性关系进行分析。采集缺血性中风病患者的相关资料，建立数据库，进行统计分析。结果纳入的61例缺血性中风病患者中风痰瘀阻证患者出现频率最高，共30例；痰湿质是缺血性中风病患者易发体质，共20例；体质与证候对应关系分析显示：气虚体质患者发病后倾向表现为气虚血瘀证；痰湿体质和平和体质患者发病后倾向表现为风痰瘀阻证；证候与MMP9，IL6关系：风痰瘀阻证、气虚血瘀证患者MMP9，IL6分布经Z检验有显著统计学差异(P<005)，气虚血瘀证患者MMP9水平明显高于风痰瘀阻证患者；风痰瘀阻证患者IL6水平明显高于气虚血瘀证患者；证候、体质与MTHFR基因多态性关系：61个样本中，共检测到杂合突变34例；纯和突变15例；无突变12例，该基因位点突变率是未突变患者的408倍；痰湿质患者发病后其MTHFR C677T基因型倾向于表现为CT基因型；风痰瘀阻证患者发病后易表现为TT基因型；阴虚动风证患者发病后易发生杂和突变，表现为CT基因型。从缺血性中风病患者证候与体质关系分析显示，痰湿质、平和质患者发病后易表现为风痰瘀阻证；气虚质患者发病后易表现为气虚血瘀证。提示发病前如对于偏颇体质进行调理，或许对预防缺血性中风病有一定效果；从缺血性中风病患者证候与MMP9，IL6关系分析显示，风痰瘀阻证与IL6水平具有相关性，气虚血瘀证与MMP9水平具有相关性。从缺血性中风病患者证候与MTHFR基因多态性关系分析显示，TT基因型患者发病后易表现为风痰瘀阻证，CT基因型患者发病后易表现为阴虚动风证；从缺血性中风病患者体质与MTHFR基因多态性关系分析显示，CT基因型患者易表现为痰湿质。为更深入地认识缺血性中风病病机提供依据，为临床治疗与预防提供干预策略。
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[关键词]缺血性中风病； 体质； 证候； MMP9，IL6，MTHFR基因多态性
Study on relationship between constitution and syndrome of MMP9，IL6 and MTHFR gene in patients with ischemic stroke
SUN Shuailing1， XIE Yanming1， ZHANG Yin1， WANG Zhifei1， YANG Jing1*， WANG Danqiao2，JIAO Yue2， CHEN Jun3， TAO Yanhui3
(1. Institute of Basic Research in Clinical Medicine， China Academy of Chineses Medical Sciences， Beijing 100700， China；
, 百拇医药
2. Medical Experiment Center， China Academy of Chinese Medical Sciences， Beijing 100700， China；
3. Wuhan Branch， Beijing Liuhe Large Gene Technology Co.， Ltd.， Wuhan 430000， China)
[Abstract]By studying the relationship between syndromes， physique and MMP9， IL6 and MTHFR gene polymorphisms in patients with ischemic stroke，The relationship between MMP9， IL6 and MTHFR gene polymorphism was analyzed in patients with ischemic strokeThe data were collected by collecting the data of patients with ischemic stroke， and the statistical analysis was carried out Syndrome：61 cases of ischemic stroke patients with stroke phlegm stasis syndrome in patients with the highest frequency， a total of 30 cases； Physical constitution： phlegm is ischemic stroke patients prone to physical， a total of 20 cases； The analysis of the relationship between constitution and syndrome shows that the patients with qi deficiency constitution tend to show qi deficiency and blood stasis syndrome after onset， The analysis of the relationship between constitution and syndrome shows that the patients with qi deficiency constitution tend to show qi deficiency and blood stasis syndrome after onset， Phlegm constitution and physical condition after the onset of symptoms tend to wind phlegm stasis syndrome； Syndrome and MMP9， IL6 relationship：The distribution of MMP9 and IL6 in patients with qi and phlegm stasis syndrome and qi deficiency and blood stasis syndrome was significantly different from that in Z test (P<005). The level of MMP9 in patients with qi deficiency and blood stasis syndrome was significantly higher than that in patients with wind phlegm and blood stasis syndrome；The level of IL6 in patients with phlegm and blood stasis syndrome was significantly higher than that in patients with qi deficiency and blood stasis syndrome Syndrome， constitution and MTHFR gene polymorphism： among the 61 samples， 34 were heterozygous mutations， 15 were pure and mutated， 12 had no mutation， The mutation rate of this locus was 408 times that of patients without mutationsThe genotype of MTHFR C677T in patients with phlegm dampness tends to be CT genotype Wind phlegm stasis syndrome in patients with easy to appear after the TT genotype； Yin deficiency syndrome in patients prone to miscellaneous and mutations， the performance of CT genotype； Analysis of the relationship between syndromes and physique in patients with ischemic stroke，Phlegm and dampness， flat quality patients after the onset of easy to show the wind phlegm stasis syndrome； Qi deficiency after the onset of symptoms in patients with Qi and blood stasis Suggesting that before the onset of such as for the partial physical conditioning， may be on the prevention of ischemic stroke have a certain effect； Analysis of the relationship between syndromes and MMP9 and IL6 in patients with ischemic stroke， Wind phlegm stasis syndrome and IL6 levels are related， Qi deficiency and blood stasis syndrome and MMP9 levels are related Analysis of the relationship between syndromes and MTHFR gene polymorphism in patients with ischemic stroke， TT genotype after the onset of symptoms prone to wind phlegm stasis syndrome， CT genotype patients after the onset of easy manifestations of Yin deficiency wind syndrome； Analysis of the relationship between physique and MTHFR gene polymorphism in patients with ischemic stroke， CT genotype is easy to show phlegmFor more indepth understanding of pathogenesis of ischemic stroke to provide the basis， For the clinical treatment and prevention to provide intervention strategies., 百拇医药(孙帅玲 谢雁鸣 张寅王志飞杨靖王丹巧焦玥陈军陶艳会)
, http://www.100md.com
[关键词]缺血性中风病； 体质； 证候； MMP9，IL6，MTHFR基因多态性
Study on relationship between constitution and syndrome of MMP9，IL6 and MTHFR gene in patients with ischemic stroke
SUN Shuailing1， XIE Yanming1， ZHANG Yin1， WANG Zhifei1， YANG Jing1*， WANG Danqiao2，JIAO Yue2， CHEN Jun3， TAO Yanhui3
(1. Institute of Basic Research in Clinical Medicine， China Academy of Chineses Medical Sciences， Beijing 100700， China；
, 百拇医药
2. Medical Experiment Center， China Academy of Chinese Medical Sciences， Beijing 100700， China；
3. Wuhan Branch， Beijing Liuhe Large Gene Technology Co.， Ltd.， Wuhan 430000， China)
[Abstract]By studying the relationship between syndromes， physique and MMP9， IL6 and MTHFR gene polymorphisms in patients with ischemic stroke，The relationship between MMP9， IL6 and MTHFR gene polymorphism was analyzed in patients with ischemic strokeThe data were collected by collecting the data of patients with ischemic stroke， and the statistical analysis was carried out Syndrome：61 cases of ischemic stroke patients with stroke phlegm stasis syndrome in patients with the highest frequency， a total of 30 cases； Physical constitution： phlegm is ischemic stroke patients prone to physical， a total of 20 cases； The analysis of the relationship between constitution and syndrome shows that the patients with qi deficiency constitution tend to show qi deficiency and blood stasis syndrome after onset， The analysis of the relationship between constitution and syndrome shows that the patients with qi deficiency constitution tend to show qi deficiency and blood stasis syndrome after onset， Phlegm constitution and physical condition after the onset of symptoms tend to wind phlegm stasis syndrome； Syndrome and MMP9， IL6 relationship：The distribution of MMP9 and IL6 in patients with qi and phlegm stasis syndrome and qi deficiency and blood stasis syndrome was significantly different from that in Z test (P<005). The level of MMP9 in patients with qi deficiency and blood stasis syndrome was significantly higher than that in patients with wind phlegm and blood stasis syndrome；The level of IL6 in patients with phlegm and blood stasis syndrome was significantly higher than that in patients with qi deficiency and blood stasis syndrome Syndrome， constitution and MTHFR gene polymorphism： among the 61 samples， 34 were heterozygous mutations， 15 were pure and mutated， 12 had no mutation， The mutation rate of this locus was 408 times that of patients without mutationsThe genotype of MTHFR C677T in patients with phlegm dampness tends to be CT genotype Wind phlegm stasis syndrome in patients with easy to appear after the TT genotype； Yin deficiency syndrome in patients prone to miscellaneous and mutations， the performance of CT genotype； Analysis of the relationship between syndromes and physique in patients with ischemic stroke，Phlegm and dampness， flat quality patients after the onset of easy to show the wind phlegm stasis syndrome； Qi deficiency after the onset of symptoms in patients with Qi and blood stasis Suggesting that before the onset of such as for the partial physical conditioning， may be on the prevention of ischemic stroke have a certain effect； Analysis of the relationship between syndromes and MMP9 and IL6 in patients with ischemic stroke， Wind phlegm stasis syndrome and IL6 levels are related， Qi deficiency and blood stasis syndrome and MMP9 levels are related Analysis of the relationship between syndromes and MTHFR gene polymorphism in patients with ischemic stroke， TT genotype after the onset of symptoms prone to wind phlegm stasis syndrome， CT genotype patients after the onset of easy manifestations of Yin deficiency wind syndrome； Analysis of the relationship between physique and MTHFR gene polymorphism in patients with ischemic stroke， CT genotype is easy to show phlegmFor more indepth understanding of pathogenesis of ischemic stroke to provide the basis， For the clinical treatment and prevention to provide intervention strategies., 百拇医药(孙帅玲 谢雁鸣 张寅王志飞杨靖王丹巧焦玥陈军陶艳会)