杜剑波

    【摘 要】目的 探讨再生美Wnt-5ARor2信号通路的软骨细胞增殖方法。方法 配置软骨细胞的培养基，制备与培养软骨细胞，分析不同浓度细胞增殖制剂对软骨细胞生长的影响，同时添加两种增殖制剂对软骨细胞生长的影响，采用WESTERN检测芦荟大黄素和咖啡酸对于细胞增殖的信号途径。结果 将相应浓度的芦荟大黄素及咖啡酸添加培养基中能够促进软骨细胞的培养，其增殖效果優于其他培养基。结论 再生美能够通过对Wnt-5ARor2信号通路进行抑制的途径调控IL-1β诱导软骨细胞增殖及再生。

    【关键词】再生美；Wnt-5ARor2信号通路；软骨细胞增殖

    中图分类号：R68 文献标识码：A 文章编号：1004-4949(2023)02-0011-03

    Chondrocyte Proliferation Method of REBORN Wnt-5ARor2 Signaling Pathway

    DU Jian-bo

    (Regenerative Beauty Medical Technology Development Co.， Ltd.， Shanghai 200000， China)

    【Abstract】Objective To investigate the method of chondrocyte proliferation in REBORN Wnt-5ARor2 signaling pathway. Methods The culture medium of chondrocytes was prepared to prepare and culture chondrocytes. The effects of different concentrations of cell proliferation preparations on the growth of chondrocytes were analyzed. At the same time ......