HPLC法测定蒙成药阿那日-6中胡椒碱的含量
【摘 要】建立蒙成药阿那日-6含量测定方法。采用高效液相色谱法测定胡椒碱的含量,色谱柱为DiamonsilC18 柱,流动相为甲醇-水(75:25),检测波长为343nm。胡椒碱的线性范围为0.0381~0.762μg,范围内呈良好的线性关系(r=0.99999),平均加样回收率为97.4%,R SD为1.5%,该方法具有简便、快速、准确等特点,可用于本品的质量控制。【关键词】HPLC;阿那日-6;胡椒碱;含量
【中图分类号】R 284.1 【文献标识码】A 【文章编号】1004- 7484(2012)04- 0339- 02
【ABSTRACT】objeetive:A HPLC method waw established for Content determination of Hydroxysafflor yellow A of prepared Mongolia traditional medicine, anari-six.Methods:A Diamonsil C18 column was used,the mobile phase was methand-water (75:25),the detection wavelength was 343nm.The Linear range was within 0.0381~0.762μg, r=0.99999.The average recovery rate was 97.4% and RSD was 1.5%.The method is simple,rapid and accurate.It can be be used for the quality control of anari-six.
【KEY WORDS】HPLC; anari-six;piperin;determination
阿那日-6由诃子、木香、石榴、干姜、白胡椒和银朱六味药组成的复方制剂 ......
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