壬基酚对Ｐ450ａｒｏｍ和ＭＩＳ基因表达的影响(3)

http://www.100md.com 2006年9月28日 杨 隽 仲伟鉴 安达龙太 北野健 安部真一

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    本研究中，在27℃雄性化水温中，用含雌二醇(E2)饲料喂养比目鱼幼鱼，仍可以完全诱导它们雌性化，并且在同一个生殖腺中P450arom的mRNA表达被诱导而MIS的mRNA表达受到抑制。而NP对27℃水温中幼鱼的雌性化作用的同时也伴随着这一现象，这提示了NP干扰性分化的作用机制很可能与雌激素一样，先通过调节P450arom和MIS mRNA的表达而发挥雌性化作用。这也提示了P450arom和MIS两个基因可以作为鱼类接触壬基酚的早期反应预警标志物，尽早预防它对人体尤其婴幼儿生殖发育系统的危害。今后可以在NP对P450arom和MIS两个基因的转录调节途径分子机制方面作更深入的研究。

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