大肠杆菌O157︰H7实时荧光PCR快速检测方法的建立(3)
本实验建立的TaqMan PCR扩增大肠杆菌O157:H7 -rfbE基因来检测大肠杆菌O157:H7的方法不仅灵敏性高、特异性好、检测周期短、检测结果准确、稳定，而且操作简便，可应用于食品微生物的快速检验，而且成本低，具有较大的推广及应用价值。
4 参考文献
[1] Griffin PM, RV Tauxe.The epidemlology of infections caused by Esehriehia Coli O157：H7. other enterohemorrhagic E.coli，and the associated hemolytic uremic syndrome[J].Epidemiol Rev，1991,13(1)：60-98.
[2] Ony J，Zhe L，Robins Browne R.Prevalence of verocytotoxigenieEshehefiehia coil serotype Ol，I：H7 in children with diarrhoea attendingaSydney hospital[J].Jpaediatr Child Health，1993。29(3)：185—187.
[3] 徐建国.一种出血性大肠杆菌的PCR检测方法[J].中华医学杂志，1995，l8(2)：225.
[4] 王崇玉.O157:H7病原体及临床检测技术的应用[J].中华医学丛刊杂志，2002，ll(2)：33.
[5]Livak KJ,Flood SAJ,Marmaro J, et al. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization PCR. Methods Applicat, 1995,4:357-362.
[6J Mckee—ML，Obriem—AD，Lnvestlgation of enterohemorrhagic E~herichiacoli O157:H7 adherence eharaeterlstics and invasion potential reveals a llew attachment patten ahered by intestinal E.coli[J].Infect Immun，1995。63(5)：2070—2074.
[7] L J R vanelden, M nijhuis. Simultaneous Detection of Influenza Viruses A and B using Real-time Quantitative PCR[J].clinical Micromology,2001,196-200.
资助项目：湖州市科技局资助项目(2007YS15)
作者简介：徐德顺(1967—)，男，主管技师，学士。(收稿日期：2008-12-02), 百拇医药(徐德顺 沈月华 程平庆)
4 参考文献
[1] Griffin PM, RV Tauxe.The epidemlology of infections caused by Esehriehia Coli O157：H7. other enterohemorrhagic E.coli，and the associated hemolytic uremic syndrome[J].Epidemiol Rev，1991,13(1)：60-98.
[2] Ony J，Zhe L，Robins Browne R.Prevalence of verocytotoxigenieEshehefiehia coil serotype Ol，I：H7 in children with diarrhoea attendingaSydney hospital[J].Jpaediatr Child Health，1993。29(3)：185—187.
[3] 徐建国.一种出血性大肠杆菌的PCR检测方法[J].中华医学杂志，1995，l8(2)：225.
[4] 王崇玉.O157:H7病原体及临床检测技术的应用[J].中华医学丛刊杂志，2002，ll(2)：33.
[5]Livak KJ,Flood SAJ,Marmaro J, et al. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization PCR. Methods Applicat, 1995,4:357-362.
[6J Mckee—ML，Obriem—AD，Lnvestlgation of enterohemorrhagic E~herichiacoli O157:H7 adherence eharaeterlstics and invasion potential reveals a llew attachment patten ahered by intestinal E.coli[J].Infect Immun，1995。63(5)：2070—2074.
[7] L J R vanelden, M nijhuis. Simultaneous Detection of Influenza Viruses A and B using Real-time Quantitative PCR[J].clinical Micromology,2001,196-200.
资助项目：湖州市科技局资助项目(2007YS15)
作者简介：徐德顺(1967—)，男，主管技师，学士。(收稿日期：2008-12-02), 百拇医药(徐德顺 沈月华 程平庆)