Treg\IL-10\IL-6对狼疮性肾炎的影响(1)
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2011年2月1日
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【摘要】目的 检测活动期、静止期狼疮性肾炎(lupus nephritis，LN)患者及正常人外周血IL-10、IL-6、CD4+CD25+CD127lo调节性T细胞(Tregs)水平，探讨Treg、IL-10 、IL-6对狼疮性肾炎的的影响。方法 采用三色荧光染色通过流式细胞仪(FCM)分析外周血中CD4+CD25+CD127lo细胞水平，用CBA 流式蛋白分析系统检测外周血中IL-10、IL-6水平。结果 1、活动期LN患者外周血Tregs低于静止期(P<0.05)；活动期LN患者外周血Treg低于正常组(P<0.01)；静止期LN患者外周血Treg低于正常组(P<0.05)。2、活动期LN患者 IL-10、IL-6与静止期相比显著升高(P<0.01)。3、LN患者Treg与IL-10、IL-6、SLEDAI之间呈负相关(P<0.05)。4、LN患者外周血IL-10、IL-6与SLEDAI呈正相关(P<0.01)。结论 1、LN患者外周血中Treg及IL-10水平的变化，可作为狼疮活动性的指标。2、LN患者外周血中Treg与IL-10、IL-6呈负相关。3、Treg、IL-10、IL-6水平的变化参与了狼疮性肾炎的发病。4、激素有助于提高Treg的水平。
【关键词】系统性红斑狼疮 流式细胞术 调节性T淋巴细胞 IL-10IL-6
中图分类号：R593.242文献标识码：A文章编号：1005-0515(2011)2-001-03
Treg, IL-10, IL-6 effects on lupus nephritis
Wang Wenhua Zhen Chuansheng Chen Jing WuWenhong LiWei
(The frist Affiliated Hospital of Fujian Medical University，Fuzhou 350005)
【Abstract】Objective To explore the role of CD4+CD25+CD127loregulatory T cells and IL-10 in the pathogenesis of lupus nephritis,we detect the level of IL-10、IL-6, CD4+CD25+CD127lo regulatory T cells(Tregs) in the peripheral blood ofpatients with lupus nephritis distinguishedactive stage and inactive stage.Methods 55 patients with lupus nephritis were differentiated according tovarious disease stage (active stage N=28, inactive stage N=27 ),and 21 cases of healthy volunteers were enrolled. Three-colour flow cytometry was used to quantitate proportions of Treg cells,and the level of IL-10、IL-6 in the peripheral blood were detected by Cytometric Bead Array System.Results 1. Compared with the inactive stage of patients with lupus nephritis， the level of CD4+CD25+CD127lo Tregs from patients with active LN was lower(P<0.05),as well ascompared the normal controls(P<0.01); compared with the normal controls , the level of CD4+CD25+CD127lo Tregs from LN inactive stage patients was lower(P<0.05). 2. Compared withthe inactive stage of LN patients , the level of IL-10、IL-6 was higher(P<0.01).3. The level of CD4+CD25+CD127lo T cell was correlated negatively with the level of IL-10、IL-6 and SLEDAI (both P < 0.01). 4. SLEDAI was correlated positively with the level of IL-10、IL-6 (P < 0.01). Conclusions 1.The level changes of Treg and IL-10 in the peripheral blood of LN could be used as the index of lupus’s reactiveness ......
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