脑卒中急性期外周血T淋巴细胞Ag-NORs的动态变化(1)
 |
| 第1页 |
参见附件(3256KB，3页)。
【摘要】目的 通过测定外周血T淋巴细胞核仁相关蛋白(Ag-NORs) 的水平，研究脑卒中患者细胞免疫功能变化。方法 选择72小时内首次发病的脑卒中患者，于发病72小时内、第14-15天抽血，健康对照组抽血检测1次，对外周血T淋巴细胞进行染色分析，以核仁银染面积与细胞核面积比值(I.S%)作为Ag-NORs检测指标。应用SPSS13.0软件进行统计分析，计量资料两组间比较采用t检验；多组间比较采用方差分析F检验和q检验。结果 1、脑卒中患者急性期外周血T淋巴细胞Ag-NORs水平(I.S%)明显降低(P<0.01)，发病后第14-15d Ag-NORs水平较72h内高(P<0.01)，但仍低于健康对照组(P<0.01)。2、脑梗死发病后同一时期重度患者外周血T淋巴细胞Ag-NORs水平较轻度患者低(P<0.05)。结论 脑卒中急性期细胞免疫功能低下，脑梗死发病后细胞免疫功能可能与病情程度有关。
【关键词】脑卒中 细胞免疫 Ag-NORs
中图分类号：R743.3 文献标识码：A 文章编号：1005-0515(2012)3-051-02
Changes of Ag-NORs in peripheral blood T lymphocytes
in patients with acute cerebral stroke
【Abstract】Objective To study the changes of Ag-NORs in peripheral blood T lymphocytes in patients with acute cerebral stroke. we hope to understand the cellular immunity after stroke onsets.Methods Patients with first-ever acute stroke within the first 72 hours after stroke onset were included. Thirty control subjects, matched for age and sex were enrolled. Subjects with inflammatory or infectious diseases, cancer, immunologic disease, or severe renal or liver failure were not included in the study. Patients who took chemotherapy, radiotherapy, operation, biological agent or immuno- suppressive drugs (within 1 month before admission) and complicated secondary infection were also excluded. The ratios of nucleolar areas by silver staining to nuclear areas (I.S%) were the index of Ag-NORs. SPSS 13.0 software was adopted to carry out the data processing and analyses. T test was adopted to carry out the comparison of the two groups. Results 1). As compared with the controls, the levels of Ag-NORs of T lymphocytes in patients with acute cerebral stroke were decreased (P<0.01), the levels of Ag-NORs within 72 hours were higher than that in the second week (P<0.01), but were still lower than that in control group (P<0.01). 2). The levels of Ag-NORs of T lymphocytes in severe patients with cerebral infarction were lower than that in slight patients in the same period (P<0.05). Conclusions The cellular immune functions in patients with acute cerebral stroke decreased, and may be associated with the pathogenetic condition in patients with ischemic stroke ......
您现在查看是摘要介绍页，详见PDF附件(3256KB，3页)。