反义hTERT寡核苷酸对人舌鳞癌细胞凋亡的影响(1)
 |
| 第1页 |
参见附件。
【摘要】目的:将反义hTERT基因导入口腔鳞癌Tca8113细胞,观察其对细胞生物学行为的影响,探讨hTERT作为靶点治疗舌癌的可行性。方法:以正义寡核苷酸组、空载体组各浓度组和空白培养基组Tca8113细胞为对照,通过MTT试验、细胞DNA ladder检测、逆转录聚合酶链反应检测、细胞凋亡检测、细胞周期检测、统计学分析反义hTERT寡核苷酸对人舌鳞癌细胞凋亡的影响。结果:实验组与对照组之间细胞凋亡率、细胞周期S期比率均有统计学意义(p<0.05),对照组之间没有统计学意义(p>0.05)。结论:hTERT的反义寡核苷酸能明显抑制Tca8113细胞增殖和诱导细胞凋亡。
【关键词】鳞状细胞癌;端粒酶逆转录酶(hTERT);Tca8113细胞;细胞凋亡
The effect of antisense oligodeoxynucleotides against hTERT on apoptosis of human tongue squamous cell carcinoma
LIU Zhihui1CHEN Shunyue1 WANG Huiming2
【Abstract】Objective: To investigate the effect of antisense oligodeoxynucleotides against hTERT on apoptosis of human tongue squamous cell carcinoma.Method: Tca8113 cells in culture medium were treated with different concentrations of antisense oligedeoxynucleotide of hTERT, were detectd by MTT assay, FCM analysis, DNA ladder test, RT-PCR. Sense oligedeoxynucleotide ofhTERT, blank carrier, and blank cuture medium respectively were served as control groups. Results: The P values of the ratio of cell apoptosis and the S phase of cell cycle were less than 0.05 between the experimental group and control groups, both of the P valueswere considered significant (p<0.05), while the P values of control groups are more than 0.05. Conclusion: The antisense oligodeoxynucleotides against hTERT have the significant inhibition growth and induction of cell apoptosis ofhuman tongue squamous cell carcinoma.
【Key words】squamous cell carcinoma, human telomerase reverse transcriptase subunit(hTERT) , Tca8113 cell line, cell apoptosis
【中图分类号】R44.6【文献标识码】B【文章编号】1005-0515(2010)009-0001-03
端粒酶是肿瘤发生、发展关键步骤。其中,端粒酶逆转录酶基因又称端粒酶催化亚基(human telomerase reverse transcriptase,hTERT)基因作为端粒酶三大组分之一,是决定端粒酶活性的关键性分子,它在端粒酶活性失控、肿瘤形成中起重要作用。国内外采用反义hTERT或(和)反义端粒酶RNA(telomerase RNA,hTR)技术逆转了淋巴瘤细胞、卵巢癌细胞、腺样囊性癌细胞恶性表型[1~5]。但目前关于反义hTERT抑制舌癌细胞增殖、诱导凋亡报道较少。本实验采用脂质体作介导,用端粒酶逆转录酶基因作为分子靶点,将反义hTERT基因导入口腔鳞癌Tca8113细胞,观察其对细胞生物学行为的影响,探讨hTERT作为靶点治疗舌癌的可行性。
1 材料与方法
实验材料。
1.1 实验细胞株。人舌鳞状细胞癌细胞系Tca8113细胞,由上海第二医科大学附属第九人民医院口腔生物研究室建系并提供。
1.2 实验主要试剂及药品。RPMI1640培养基(美国GibcoBRL公司),特级小牛血清(杭州四季青生物工程公司)。小量凋亡DNA抽提纯化试剂盒(上海华舜生物工程有限公司) ......
您现在查看是摘要介绍页，详见PDF附件(2610kb)。