咳喘宁口服液对支气管哮喘大鼠PI3K信号转导途径的影响(1)
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摘要：目的 观察咳喘宁口服液对呼吸道合胞病毒诱发支气管哮喘(以下简称“哮喘”)大鼠CD4+、CD8+T细胞内磷脂酰肌醇-3-激酶(PI3K)-110α表达的影响，探讨其防治病毒诱发哮喘的机理。方法 将SD大鼠随机分为磷酸盐缓冲液(PBS)对照组、模型组、地塞米松+沙丁胺醇组(阳性药组)和咳喘宁组。建立卵白蛋白哮喘大鼠模型，并以呼吸道合胞病毒激发哮喘。采用磁珠结合法分离CD4+、CD8+T细胞，使用流式细胞仪检测T细胞内PI3K-110α的表达水平。结果 模型组大鼠CD4+、CD8+T细胞百分率及CD4+、CD8+T细胞内PI3K-110α的表达水平显著高于PBS对照组、阳性药组及咳喘宁组，差异有统计学意义(均P<0.01)。经Pearson相关分析，各组大鼠CD4+、CD8+T细胞百分率与CD4+、CD8+T细胞内表达的PI3K-110α的表达水平无明显相关性。结论 CD4+、CD8+T细胞参与了哮喘免疫平衡调节，其表达的PI3K在病毒诱发哮喘模型中明显增高。咳喘宁口服液能抑制CD4+、CD8+T细胞内PI3K的表达，可能是其治疗哮喘的免疫机制之一。
关键词：支气管哮喘；磷脂酰肌醇-3-激酶信号转导；咳喘宁口服液；细胞培养；大鼠
DOI：10.3969/j.issn.1005-5304.2013.07.012
中图分类号：R285.5 文献标识码：A 文章编号：1005-5304(2013)07-0030-03
Effect of Kechuanning Oral Liquid on Signal Transduction Pathway of PI3K in Asthma Rats Induced by Virus LUO Yin-he1， LI Hua1， YANG Jing-yi2， WANG Meng-qing2， HUANG Sheng2， HUANG Ting2， JIANG Ping2， JIAO Luo-jia1， LI Ying1 (1.Hunan University of TCM， Changsha 410208， China；2.The First Affiliated Hospital of Hunan University of TCM， Changsha 410007， China)
Abstract：Objective To observe the effect of Kechuanning Oral liquid on the expression of P13K in CD4+ and CD8+T cells of asthma rats induced by respiratory syncytial virus (RSV)， and explore its mechanism of preventing and treating asthma induced by virus. Methods SD rats were randomly divided into phosphate-buffered saline (PBS) control group， model group， dexamethasone and salbutamol group (positive drug group)， and Kechuanning oral liquid group. Ovalbumin asthma rats model was built and induced by RSV. CD4+ and CD8+T cells were separated by magnetic beads method. The expressive level of PI3K-110α in T cells was detected by immumofluorescence method with flow cytometry. Results Compared with PBS control group， positive drug group and Kechuanning oral liquid group， the CD4+ and CD8+T cells percentage and the expression level of PI3K-110α in CD4+ and CD8+T cells of model group were significantly higher. The differences were statistically significant (P<0.01). The CD4+ and CD8+T cells percentage was not associated with the expression level of PI3K-110α in CD4+ and CD8+T cells of each group by Pearson correlation analysis. Conclusion The CD4+ and CD8+T cells involve in the asthma immunity balanced modulation， and the level of PI3K expressed by CD4+ and CD8+T cells increase distinctly. Kechuanning oral liquid can restrain the expression of PI3K in CD4+ and CD8+T cells， which might be one of the immunologic mechanisms of treating asthma.
Key words：bronchial asthma；P13K signal transduce；Kechuanning oral liquid；cell culture；rats
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