癫痫清颗粒中α—细辛醚含量测定方法研究
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摘要：目的 探讨癫痫清颗粒中α-细辛醚含量测定方法。方法 采用Kromasil C18色谱柱(250 mm×4.6 mm， 5 μm)，以甲醇(A)-水(B)为流动相，梯度洗脱，流速为1 mL/min，紫外检测器检测波长为257 nm，柱温为25 ℃。结果 采用70%乙醇回流提取20 min制备供试品溶液，α-细辛醚在进样量1.24×10-3～7.44×10-3 μg范围内与峰面积呈良好的线性关系(r=0.999 5)，精密度、稳定性、重复性均较好，平均加样回收率为98.14%，RSD=2.5%。结论 本试验为中药复方制剂中α-细辛醚的测定提供了准确、快速的分析方法。
关键词：癫痫清颗粒；α-细辛醚；高效液相色谱法；含量测定
Abstract：Objective To discuss the content determination method of α-asarone in Dianxianqing Granules. Methods Kromasil C18 column (250 mm×4.6 mm， 5 μm) was used， with methanol and water as mobile phase of gradient elution. The flow rate was 1.0 mL/min， and the detection wavelength was 257 nm. The column temperature was 25 ℃. Results The sample solution was prepared by reflux extracted with 70% ethanol for 20 min. α-asarone had a good linearity in the ranges of 1.24×10-3-7.44×10-3 μg (r=0.999 5). The method was quite good in precision， stability and repeatability ......
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