HPLC—DAD同时测定黄芪生脉饮中4种成分含量(1)
摘要:目的 采用高效液相色谱法建立同时测定黄芪生脉饮中五味子乙素、五味子酯甲、党参炔苷和鲁斯可皂苷元含量的方法。方法 色谱柱:Agilent Eclipse C18(4.6 mm×250 mm,5 μm);柱温:30 ℃;流动相:乙腈-0.1%冰醋酸水溶液,梯度洗脱;流速:1.0 mL/min;检测波长:280 nm。结果 五味子乙素、五味子酯甲、党参炔苷和鲁斯可皂苷元的标准曲线线性关系良好,线性范围分别为0.862 5~21.562 5 μg(r2=0.999 1)、0.737 5~18.437 5 μg(r2=0.999 4)、0.095 2~2.380 0 μg(r2=0.999 6)、0.810 0~20.250 0 μg(r2=0.999 0),平均回收率分别为98.06%、99.61%、97.98%、99.30%,RSD分别为1.64%、2.72%、1.45%、1.25%。结论 该法准确可靠、快速、专属性强、结果稳定、重复性好。
关键词:黄芪生脉饮;五味子乙素;五味子酯甲;党参炔苷;鲁斯可皂苷元;高效液相色谱法
, 百拇医药
DOI:10.3969/j.issn.1005-5304.2015.07.027
中图分类号:R284.1 文献标识码:A 文章编号:1005-5304(2015)07-0096-04
Simultaneous Determination of Four Components in Huangqi Shengmai Decoction by HPLC-DAD LU Cheng1,2, ZHOU Sheng-hui1,2, LI Wei2, ZHENG Xiu-li2, GAO Qing-jian1,2, TANG Hao2 (1.School of Pharmacy, Ningxia Medical University, Yinchuan 750000, China;2.Gansu Provincial Hospital, Lanzhou 730000, China)
Abstract:Objective To establish a method for simultaneous determination of contents of schisandrin B, schisantherin A, lobetyolin and ruscogenin in Huangqi Shengmai Decoction with HPLC method. Methods The chromatographic column was an Agilent Eclipse C18 column (4.6 mm × 250 mm, 5 μm) that was maintained at a constant temperature of 30 ℃. The mobile phase was 0.1% acetic acid solution-acetonitrile, with gradient elution. The flow rate was 1.0 mL/min. The detection was at wavelength of 280 nm. Results The standard curves of schisandrin B, schisantherin A, lobetyolin and ruscogenin had good linearity in the ranges of 0.862 5-21.562 5 μg (r2=0.999 1), 0.737 5-18.437 5μg (r2=0.999 4), 0.095 2-2.380 0 μg (r2=0.999 6), and 0.810 0- 20.250 0 μg (r2=0.999 0), respectively. The average recoveries of the four components were 98.06% (RSD=1.64%), 99.61% (RSD=2.72%), 97.98% (RSD=1.45%), and 99.30% (RSD=1.25%), respectively. Conclusion This method is accurate, rapid and specific, achieving the stable results with high reproducibility.
, 百拇医药
Key words:Huangqi Shengmai Decoction;schisandrin B;schisantherin A;lobetyolin;ruscogenin;HPLC
黄芪生脉饮由黄芪、党参、麦冬、五味子、南五味子5味药组成,是由古方生脉散演变而来。生脉散处方源自张元素《医学启源》,具有益气滋阴、养心补肺功效[1]。目前已有大量关于黄芪生脉饮临床应用的文献报道[2-3]。2010年版《中华人民共和国药典》(一部)[4]记载,黄芪甲苷、五味子乙素、五味子酯甲、党参炔苷、鲁斯可皂苷元分别是黄芪、五味子、南五味
通讯作者:汤浩,E-mail:gsyy_th@sina.cn
子、党参和麦冬的主要有效成分。
目前,关于黄芪生脉饮中有效成分的测定多集中于黄芪甲苷[5-6],尚未见对黄芪生脉饮中其余有效成分同时进行定量分析的文献报道。《中华人民共和国卫生部药品标准·中药成方制剂(第十六册)》仅有对黄芪生脉饮相对密度的测定,而无对该制剂有效成分的定量测定项[7]。因此,本试验采用高效液相色谱法(HPLC)同时测定黄芪生脉饮中五味子乙素、五味子酯甲、党参炔苷和鲁斯可皂苷元的含量,为该制剂质量标准的完善及医院中成药制剂引进的质量测评提供参考。, 百拇医药(陆铖 周盛会 李炜 郑修丽 高庆剑 汤浩)
关键词:黄芪生脉饮;五味子乙素;五味子酯甲;党参炔苷;鲁斯可皂苷元;高效液相色谱法
, 百拇医药
DOI:10.3969/j.issn.1005-5304.2015.07.027
中图分类号:R284.1 文献标识码:A 文章编号:1005-5304(2015)07-0096-04
Simultaneous Determination of Four Components in Huangqi Shengmai Decoction by HPLC-DAD LU Cheng1,2, ZHOU Sheng-hui1,2, LI Wei2, ZHENG Xiu-li2, GAO Qing-jian1,2, TANG Hao2 (1.School of Pharmacy, Ningxia Medical University, Yinchuan 750000, China;2.Gansu Provincial Hospital, Lanzhou 730000, China)
Abstract:Objective To establish a method for simultaneous determination of contents of schisandrin B, schisantherin A, lobetyolin and ruscogenin in Huangqi Shengmai Decoction with HPLC method. Methods The chromatographic column was an Agilent Eclipse C18 column (4.6 mm × 250 mm, 5 μm) that was maintained at a constant temperature of 30 ℃. The mobile phase was 0.1% acetic acid solution-acetonitrile, with gradient elution. The flow rate was 1.0 mL/min. The detection was at wavelength of 280 nm. Results The standard curves of schisandrin B, schisantherin A, lobetyolin and ruscogenin had good linearity in the ranges of 0.862 5-21.562 5 μg (r2=0.999 1), 0.737 5-18.437 5μg (r2=0.999 4), 0.095 2-2.380 0 μg (r2=0.999 6), and 0.810 0- 20.250 0 μg (r2=0.999 0), respectively. The average recoveries of the four components were 98.06% (RSD=1.64%), 99.61% (RSD=2.72%), 97.98% (RSD=1.45%), and 99.30% (RSD=1.25%), respectively. Conclusion This method is accurate, rapid and specific, achieving the stable results with high reproducibility.
, 百拇医药
Key words:Huangqi Shengmai Decoction;schisandrin B;schisantherin A;lobetyolin;ruscogenin;HPLC
黄芪生脉饮由黄芪、党参、麦冬、五味子、南五味子5味药组成,是由古方生脉散演变而来。生脉散处方源自张元素《医学启源》,具有益气滋阴、养心补肺功效[1]。目前已有大量关于黄芪生脉饮临床应用的文献报道[2-3]。2010年版《中华人民共和国药典》(一部)[4]记载,黄芪甲苷、五味子乙素、五味子酯甲、党参炔苷、鲁斯可皂苷元分别是黄芪、五味子、南五味
通讯作者:汤浩,E-mail:gsyy_th@sina.cn
子、党参和麦冬的主要有效成分。
目前,关于黄芪生脉饮中有效成分的测定多集中于黄芪甲苷[5-6],尚未见对黄芪生脉饮中其余有效成分同时进行定量分析的文献报道。《中华人民共和国卫生部药品标准·中药成方制剂(第十六册)》仅有对黄芪生脉饮相对密度的测定,而无对该制剂有效成分的定量测定项[7]。因此,本试验采用高效液相色谱法(HPLC)同时测定黄芪生脉饮中五味子乙素、五味子酯甲、党参炔苷和鲁斯可皂苷元的含量,为该制剂质量标准的完善及医院中成药制剂引进的质量测评提供参考。, 百拇医药(陆铖 周盛会 李炜 郑修丽 高庆剑 汤浩)