蒙药孟根乌苏(水银)—18味丸对大鼠肾脏毒性初步研究(1)
摘要：目的 观察孟根乌苏(水银)-18味丸对大鼠肾脏功能的影响，探讨其肾毒性。方法 将Wistar雄性大鼠根据体质量随机分为8组：正常对照组，孟根乌苏-18味丸低、中、高剂量组，孟根乌苏炮制品组，硫化汞组，氯化汞组，氯化亚汞组，每组10只。各组大鼠给予相应药物灌胃，连续15 d。分别检测大鼠血清尿素(UREA)、肌酐(CREA)水平，观察肾组织形态学变化，并用电感耦合等离子体发射光谱仪和电感耦合等离子体质谱法检测肾汞蓄积量，原位末端标记法检测肾细胞凋亡，免疫组化检测肾Ⅲ型胶原蛋白表达，实时荧光定量PCR检测肾脏金属硫蛋白(MT)-1、MT-2基因表达。结果 氯化汞、氯化亚汞大鼠肾脏指数比正常对照组和孟根乌苏炮制品组显著升高(P<0.01)；各组大鼠血清CREA和UREA含量无显著差异。肾脏病理观查结果表明，孟根乌苏-18味丸低剂量组(临床常用剂量)大鼠肾脏出现一定的病理损伤，表现为肾小球轻度肥大、肾小管上皮中度肿胀变性，孟根乌苏-18味丸中、高剂量组肾脏也出现一定损伤，硫化汞组、氯化汞组、氯化亚汞组大鼠肾病理变化更显著。与正常对照组和孟根乌苏炮制品组比较，氯化汞组和氯化亚汞组大鼠肾汞蓄积量显著升高(P<0.01)，氯化汞组、氯化亚汞组大鼠肾细胞凋亡率显著升高(P<0.01)，硫化汞组、氯化汞组、氯化亚汞组大鼠肾脏Ⅲ型胶原蛋白表达显著升高(P<0.05，P<0.01)。与正常对照组比较，氯化亚汞组大鼠肾组织中MT-1 mRNA表达显著升高(P<0.05)，氯化汞组、氯化亚汞组大鼠肾组织中MT-2 mRNA表达显著升高(P<0.05，P<0.01)；与孟根乌苏炮制品组比较，氯化亚汞组大鼠肾组织中MT-1 mRNA表达显著升高(P<0.05)。结论 临床常用剂量孟根乌苏-18味丸在15 d用药过程中未对大鼠肾脏功能造成明显的损伤。
关键词：孟根乌苏-18味丸；汞化合物；肾毒性；大鼠
DOI：10.3969/j.issn.1005-5304.2016.02.019
中图分类号：R291.2 文献标识码：A 文章编号：1005-5304(2016)02-0067-06
Preliminary Investigation of Mongolian Meng-gen-wu-su (Mercury)-18-Composition Pills on Renal Toxicity in Rats TONG Hai-ying1， FAN Ang-ran2， YU Xue2， ZHANG Yue1， WU Jisiguleng3， LI Jing4， HU Rilebagen4 (1. Institute of Ethnic Medicine， Beijing University of Chinese Medicine， Beijing 100029， China； 2. School of Basic Medical Sciences， Beijing University of Chinese Medicine， Beijing 100029， China； 3. Inner Mongolia International Mongolian Medicine Hospital， Hohhot 010020， China； 4. College of Mongolian Medicine and Pharmacy， Inner Mongolia Medical University， Hohhot 010110， China)
Abstract： Objective To study the effects of Mongolian Meng-gen-wu-su (Mercury)-18-Composition Pills on renal toxicity in rats； To discuss its Renal Toxicity. Methods Eighty male Wistar rats were randomly divided into eight groups according to the weight (10 rats in each group)： normal control group， low-， medium- and high-dose Meng-gen-wu-su-18-Composition Pills groups， Meng-gen-wu-su-18-Composition processed products group， mercuric sulfide group， mercuric chloride group and mercurous chloride group. After one week adaption， oral gavages were given to each group of rats for fifteen days. Serum UREA and CREA content and renal tissue morphological changes were detected； Mercury accumulation in kidney was determined by inductively coupled plasma optical emission spectroscopy and inductively coupled plasma source mass spectrometer； Apoptosis of renal cells was determined by TUNEL； Renal III type collagen protein expressions were determined by Immunohistochemical method； Kidney MT-1， MT-2 gene expression changes were determined by real-time fluorescence quantitative PCR. Results After, http://www.100md.com(佟海英 范盎然 于雪 张月 乌吉斯古冷 李婧 呼日乐巴根)
关键词：孟根乌苏-18味丸；汞化合物；肾毒性；大鼠
DOI：10.3969/j.issn.1005-5304.2016.02.019
中图分类号：R291.2 文献标识码：A 文章编号：1005-5304(2016)02-0067-06
Preliminary Investigation of Mongolian Meng-gen-wu-su (Mercury)-18-Composition Pills on Renal Toxicity in Rats TONG Hai-ying1， FAN Ang-ran2， YU Xue2， ZHANG Yue1， WU Jisiguleng3， LI Jing4， HU Rilebagen4 (1. Institute of Ethnic Medicine， Beijing University of Chinese Medicine， Beijing 100029， China； 2. School of Basic Medical Sciences， Beijing University of Chinese Medicine， Beijing 100029， China； 3. Inner Mongolia International Mongolian Medicine Hospital， Hohhot 010020， China； 4. College of Mongolian Medicine and Pharmacy， Inner Mongolia Medical University， Hohhot 010110， China)
Abstract： Objective To study the effects of Mongolian Meng-gen-wu-su (Mercury)-18-Composition Pills on renal toxicity in rats； To discuss its Renal Toxicity. Methods Eighty male Wistar rats were randomly divided into eight groups according to the weight (10 rats in each group)： normal control group， low-， medium- and high-dose Meng-gen-wu-su-18-Composition Pills groups， Meng-gen-wu-su-18-Composition processed products group， mercuric sulfide group， mercuric chloride group and mercurous chloride group. After one week adaption， oral gavages were given to each group of rats for fifteen days. Serum UREA and CREA content and renal tissue morphological changes were detected； Mercury accumulation in kidney was determined by inductively coupled plasma optical emission spectroscopy and inductively coupled plasma source mass spectrometer； Apoptosis of renal cells was determined by TUNEL； Renal III type collagen protein expressions were determined by Immunohistochemical method； Kidney MT-1， MT-2 gene expression changes were determined by real-time fluorescence quantitative PCR. Results After, http://www.100md.com(佟海英 范盎然 于雪 张月 乌吉斯古冷 李婧 呼日乐巴根)