溃结宁膏穴位敷贴对脾肾阳虚型溃疡性结肠炎大鼠血清及结肠组织促炎因子与抗炎因子的影响(1)
摘要：目的 观察溃结宁膏穴位敷贴对脾肾阳虚型溃疡性结肠炎(UC)大鼠血清及结肠组织促炎因子干扰素-γ(IFN-γ)、白细胞介素(IL)-12与抗炎因子IL-4、IL-10的影响，探讨其治疗脾肾阳虚型UC的作用机制。方法 采用番泻叶溶液灌胃、喷冰水刺激和三硝基苯磺酸(TNBS)乙醇法制作脾肾阳虚型UC大鼠病证结合模型。将SD大鼠随机分为空白组、模型组、敷贴组、柳氮磺胺吡啶(SASP)组，每组10只，空白组和模型组予无药物的巴布剂基质穴位敷贴加生理盐水灌胃，敷贴组予溃结宁膏穴位敷贴加生理盐水灌胃，SASP组予无药物的巴布剂基质穴位敷贴加SASP灌胃，疗程均为28 d。治疗期间观察各组大鼠一般状况，治疗后肉眼观察结肠组织大体形态，HE染色镜下观察病理形态并评分，ELISA检测各组大鼠血清及结肠组织IFN-γ、IL-12、IL-4、IL-10水平。结果 敷贴组和SASP组UC大鼠一般状况、结肠组织肉眼大体形态、病理形态均较模型组好转且评分降低；与模型组比较，敷贴组、SASP组血清和结肠组织IL-4、IL-10水平明显升高，IFN-γ、IL-12水平明显降低(P<0.01)；同时，敷贴组IL-4、IL-10下降水平低于SASP组，IFN-γ、IL-12上升水平高于SASP组，但差异无统计学意义(P>0.05)。结论 溃结宁膏可能通过部分恢复大鼠血清及结肠组织促炎因子与抗炎因子的平衡来发挥其治疗脾肾阳虚型UC的作用。
关键词：溃疡性结肠炎；溃结宁膏；穴位敷贴；干扰素-γ；白细胞介素-12；白细胞介素-4；白细胞介素-10；大鼠
DOI：10.3969/j.issn.1005-5304.2016.03.012
中图分类号：R285.5 文献标识码：A 文章编号：1005-5304(2016)03-0042-05
Abstract： Objective To observe the effects of Kuijiening Plaster point application on the levels of pro-inflammatory cytokines IFN-γ， IL-12 and anti-inflammatory cytokines IL-4， IL-10 in serum and colonic tissue of ulcerative colitis (UC) rats with spleen-kidney yang deficiency； To investigate the possible mechanism of Kuijiening for the treatment of UC with spleen-kidney yang deficiency. Methods Sennae Folium solution gavage， ice water stimulation and trinitrobenzene-sulfonic acid ethanol combinative methods were applied to establish the models of UC rats with spleen-kidney yang deficiency. Rat models were randomly divided into control group， model group， application group and SASP group， 10 in each group. The control group and model group were given medicine-free cataplasm matrix point application and perfused with NS. The application group was given Kuijiening Plaster point application and perfused with NS. SASP group was given medicine-free cataplasm matrix point application and perfused with SASP. The courses were 28 days. General conditions of rats in each group were observed during treatment. The general morphology and pathological form of colonic tissue were observed with naked eye and light microscopy through HE staining respectively in each group post-treatment. Pathological features were observed and scored by HE dyeing. Levels of IFN-γ， IL-12， IL-4 and IL-10 in serum and colonic tissue of rats in each group were detected with ELISA. Results The general conditions， gross morphology and optical microscope pathological morphology of colonic tissue improved and scores decreased in application group and SASP group. Compared with the model group， levels of IL-4 and IL-10 significantly increased， while the levels of IFN-γ and IL-12 decreased in serum and colonic tissue in application group and SASP group， with statistical significance (P<0.01). Moreover， levels of IL-4 and IL-10 in application group decreased not as much as those in SASP group， while IFN-γ and IL-12 concentration increased higher than SASP group， without statistical significance (P>0.05). Conclusion Kuijiening Plaster point application can play a role in treating UC with spleen-kidney yang deficiency through partially restoring the balance between pro- and anti-inflammatory cytokines in serum and colon tissue of rats., 百拇医药(王璇 朱莹 邹君君)
关键词：溃疡性结肠炎；溃结宁膏；穴位敷贴；干扰素-γ；白细胞介素-12；白细胞介素-4；白细胞介素-10；大鼠
DOI：10.3969/j.issn.1005-5304.2016.03.012
中图分类号：R285.5 文献标识码：A 文章编号：1005-5304(2016)03-0042-05
Abstract： Objective To observe the effects of Kuijiening Plaster point application on the levels of pro-inflammatory cytokines IFN-γ， IL-12 and anti-inflammatory cytokines IL-4， IL-10 in serum and colonic tissue of ulcerative colitis (UC) rats with spleen-kidney yang deficiency； To investigate the possible mechanism of Kuijiening for the treatment of UC with spleen-kidney yang deficiency. Methods Sennae Folium solution gavage， ice water stimulation and trinitrobenzene-sulfonic acid ethanol combinative methods were applied to establish the models of UC rats with spleen-kidney yang deficiency. Rat models were randomly divided into control group， model group， application group and SASP group， 10 in each group. The control group and model group were given medicine-free cataplasm matrix point application and perfused with NS. The application group was given Kuijiening Plaster point application and perfused with NS. SASP group was given medicine-free cataplasm matrix point application and perfused with SASP. The courses were 28 days. General conditions of rats in each group were observed during treatment. The general morphology and pathological form of colonic tissue were observed with naked eye and light microscopy through HE staining respectively in each group post-treatment. Pathological features were observed and scored by HE dyeing. Levels of IFN-γ， IL-12， IL-4 and IL-10 in serum and colonic tissue of rats in each group were detected with ELISA. Results The general conditions， gross morphology and optical microscope pathological morphology of colonic tissue improved and scores decreased in application group and SASP group. Compared with the model group， levels of IL-4 and IL-10 significantly increased， while the levels of IFN-γ and IL-12 decreased in serum and colonic tissue in application group and SASP group， with statistical significance (P<0.01). Moreover， levels of IL-4 and IL-10 in application group decreased not as much as those in SASP group， while IFN-γ and IL-12 concentration increased higher than SASP group， without statistical significance (P>0.05). Conclusion Kuijiening Plaster point application can play a role in treating UC with spleen-kidney yang deficiency through partially restoring the balance between pro- and anti-inflammatory cytokines in serum and colon tissue of rats., 百拇医药(王璇 朱莹 邹君君)