摘要：目的 建立超高效液相色谱法(UPLC)测定青藤散中青藤碱含量的方法。方法 采用UPLC，色谱柱为ACQUITY UPLC HSS C18(2.1 mm×50 mm，1.8 ?m)，流动相为乙腈-水-乙二胺(50∶50∶0.25)，流速为0.2 mL/min，进样量为2 ?L，于检测波长283 nm处对青藤碱进行含量测定。结果 出峰时间在1 min左右，青藤碱在34.2～2188.0 ng范围内线性关系良好。结论 本研究所建立的方法简便快速、稳定可靠，可用于青藤散中青藤碱的含量测定。

    关键词：青藤散；青藤碱；超高效液相色谱法；含量测定

    DOI：10.3969/j.issn.1005-5304.2016.03.024

    中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2016)03-0089-03

    Abstract： Objective To establish an UPLC method for the determination of sinomenine in Sinomenine External Applied Powder. Methods The UPLC method was carried out on a C18 column by using acetonitrile-water-ethylene diamine (50：50：0.25) as mobile phase. The flow rate was 0.2 mL/min； the sample quantity was 2 ?L； the detection wavelength was 283 nm. Results The peak time was within 1 min or so. The calibration curve of sinomenine was in the linear range of 34.2–2188.0 ng. Conclusion The method is simple ......