All items of method validation were in line with the requirements. The main pharmacokinetic parameters of paeoniflorin and paeonolide were showed as follows： Cmax of (8.50±2.01) and (4.13±0.72) ?g/mL； tmax of (10.00±1.73) and (20.00±2.11) min； t1/2 of (142.98±30.11) and (127.68±35.74) min. Conclusion The HPLC-MS/MS method established in this study is simple， feasible， accurate， reliable， highly sensitive， and specific， which can be applied to study the pharmacokinetic characteristics of paeoniflorin and paeonolide in rats in vivo.

    Keywords： HPLC-MS/MS； paeoniflorin； paeonolide； pharmacokinetics； rats

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