基于傅里叶变换红外光谱指纹特征的甘肃8个主产县红芪药材鉴别研究(1)
摘要：目的 研究甘肃8个主产县红芪药材的傅里叶变换红外光谱(FT-IR)指纹特征，为不同产地红芪的鉴别应用提供依据。方法 采用FT-IR技术，采集甘肃省8个红芪主产县110批红芪样品在4000～400 cm-1范围内的指纹图谱数据，对指纹图谱的共有模式进行光谱解析，对8个主产县红芪的FT-IR指纹图谱进行相似度分析，比较不同主产县红芪的FT-IR指纹特征。结果 8个主产县红芪FT-IR指纹图谱的平均相似度大小排序为宕昌>礼县>西和>武都>漳县>岷县>陇西>渭源，陇西、渭源红芪与其他产地的样品差异较大；陇南地区(宕昌、礼县、西和、武都)红芪的FT-IR指纹图谱较为相似，平均相似度较大，而定西地区(漳县、岷县、陇西、渭源)红芪的FT-IR指纹图谱较为相似，平均相似度较小；每个主产县红芪的FT-IR指纹图谱均具有显著、唯一的FT-IR指纹特征。结论 根据FT-IR指纹特征能够实现甘肃8个主产县红芪药材的鉴别应用。
关键词：红芪；傅里叶变换红外光谱；指纹图谱；相似度分析；指纹鉴别
DOI：10.3969/j.issn.1005-5304.2018.11.014
中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2018)11-0062-06
Abstract： Objective To study the FT-IR fingerprint characteristics of Hedysari Radix from 8 producing counties in Gansu Province； To provide references for identification and application of Hedysari Radix in different producing counties. Methods FT-IR fingerprints of 110 batches of Hedysari Radix from 8 producing counties in Gansu Province were collected in the wave number range of 4000–400 cm-1. The common pattern of the fingerprints were analyzed， and the similarity analysis were used to analyze the FT-IR fingerprints of Hedysari Radix from 8 producing counties. The FT-IR fingerprint characteristics of Hedysari Radix from 8 producing counties in Gansu Province were compared. Results The rank of average similarity of FT-IR fingerprints of Hedysari Radix from 8 producing counties was Tanchang County > Li County > Xihe County > Wudu District > Zhang County > Min County > Longxi County > Weiyuan County， and Hedysari Radix from Longxi County and Weiyuan County were very different from other producing counties. The FT-IR fingerprints of Hedysari Radix from Longnan City (Tanchang County， Li County， Xihe County and Wudu District) were similar， and the average similarity was relatively high； while that from Dingxi City (Zhang County， Min County， Longxi County and Weiyuan County) were similar， and the average similarity was relatively low. Hedysari Radix from every producing county had a significant and unique FT-IR fingerprint characteristic. Conclusion The identification and application of Hedysari Radix from 8 producing counties in Gansu Province can be realized according to FT-IR fingerprint characteristics.
Keywords： Hedysari Radix； FT-IR； fingerprint； similarity analysis； fingerprint identification
红芪是豆科岩黄芪属植物多序岩黄芪Hedysarum polybotrys Hand.-Mazz的干燥根，最早记载于《神农本草经》黄芪项下，被列为上品，入药已有2000多年历史[1]。红芪有补气固表、利尿、托毒排脓、敛疮生肌等功效[2]，在免疫调节、抗肿瘤、抗突变及抗损伤等方面作用突出[3]。
红芪是甘肃特有的道地药材，主产于甘肃陇南地区的武都、礼县、宕昌及定西地区的岷县、陇西、漳县等地，产量占全国95%以上。其中，陇南是甘肃省唯一属于长江水系并拥有亚热带气候的地区，而定西则属于中温带半干旱区。研究发现，不同时间、空间的气候条件、水土异质等环境变化均会影响药用植物的物候规律、生长发育、能量代谢、物质合成及气体交换等生理作用，继而影响其次生代谢产物的变化，从而导致药材质量及临床疗效有所差异[4]。因此，探讨不同环境因子背景下红芪药材的物质基础、遗传效应、药理作用等差异，对于突出红芪的产地比较优势、打造地域性红芪品牌具有重要的实际意义。本研究对甘肃8个主产县红芪药材的傅里叶变换红外光谱(FT-IR)指紋图谱进行相似性分析及差异比较，旨在发现甘肃不同主产县红芪的FT-IR指纹特征，为不同产地红芪的鉴别应用提供依据。, 百拇医药(顾志荣 许爱霞 祁梅 李芳 张瑛 钱倩 石磊)
关键词：红芪；傅里叶变换红外光谱；指纹图谱；相似度分析；指纹鉴别
DOI：10.3969/j.issn.1005-5304.2018.11.014
中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2018)11-0062-06
Abstract： Objective To study the FT-IR fingerprint characteristics of Hedysari Radix from 8 producing counties in Gansu Province； To provide references for identification and application of Hedysari Radix in different producing counties. Methods FT-IR fingerprints of 110 batches of Hedysari Radix from 8 producing counties in Gansu Province were collected in the wave number range of 4000–400 cm-1. The common pattern of the fingerprints were analyzed， and the similarity analysis were used to analyze the FT-IR fingerprints of Hedysari Radix from 8 producing counties. The FT-IR fingerprint characteristics of Hedysari Radix from 8 producing counties in Gansu Province were compared. Results The rank of average similarity of FT-IR fingerprints of Hedysari Radix from 8 producing counties was Tanchang County > Li County > Xihe County > Wudu District > Zhang County > Min County > Longxi County > Weiyuan County， and Hedysari Radix from Longxi County and Weiyuan County were very different from other producing counties. The FT-IR fingerprints of Hedysari Radix from Longnan City (Tanchang County， Li County， Xihe County and Wudu District) were similar， and the average similarity was relatively high； while that from Dingxi City (Zhang County， Min County， Longxi County and Weiyuan County) were similar， and the average similarity was relatively low. Hedysari Radix from every producing county had a significant and unique FT-IR fingerprint characteristic. Conclusion The identification and application of Hedysari Radix from 8 producing counties in Gansu Province can be realized according to FT-IR fingerprint characteristics.
Keywords： Hedysari Radix； FT-IR； fingerprint； similarity analysis； fingerprint identification
红芪是豆科岩黄芪属植物多序岩黄芪Hedysarum polybotrys Hand.-Mazz的干燥根，最早记载于《神农本草经》黄芪项下，被列为上品，入药已有2000多年历史[1]。红芪有补气固表、利尿、托毒排脓、敛疮生肌等功效[2]，在免疫调节、抗肿瘤、抗突变及抗损伤等方面作用突出[3]。
红芪是甘肃特有的道地药材，主产于甘肃陇南地区的武都、礼县、宕昌及定西地区的岷县、陇西、漳县等地，产量占全国95%以上。其中，陇南是甘肃省唯一属于长江水系并拥有亚热带气候的地区，而定西则属于中温带半干旱区。研究发现，不同时间、空间的气候条件、水土异质等环境变化均会影响药用植物的物候规律、生长发育、能量代谢、物质合成及气体交换等生理作用，继而影响其次生代谢产物的变化，从而导致药材质量及临床疗效有所差异[4]。因此，探讨不同环境因子背景下红芪药材的物质基础、遗传效应、药理作用等差异，对于突出红芪的产地比较优势、打造地域性红芪品牌具有重要的实际意义。本研究对甘肃8个主产县红芪药材的傅里叶变换红外光谱(FT-IR)指紋图谱进行相似性分析及差异比较，旨在发现甘肃不同主产县红芪的FT-IR指纹特征，为不同产地红芪的鉴别应用提供依据。, 百拇医药(顾志荣 许爱霞 祁梅 李芳 张瑛 钱倩 石磊)