滋阴益气活血方对免疫性卵巢早衰小鼠转化生长因子-β1及PI3K相关蛋白表达的影响(1)

http://www.100md.com 2020年1月1日 《中国中医药信息杂志》 20201

     摘要：目的 觀察滋阴益气活血方对免疫性卵巢早衰小鼠转化生长因子-β1(TGF-β1)、PI3K、AKT1蛋白表达的影响，探讨其改善卵巢功能的作用机制。方法 取Balb/C雌性小鼠60只，随机选取10只为空白组，其余50只用于造模。用透明带3作抗原，皮下多点注射制作免疫性卵巢早衰动物模型。成模后实验小鼠随机分为模型组、阳性对照组和滋阴益气活血方低、中、高剂量组，各给药组给予相应药物，连续给药30 d。HE染色观察小鼠卵巢组织病理形态，免疫组化检测小鼠卵巢组织TGF-β1、PI3K、AKT1蛋白表达。结果 与空白组比较，模型组小鼠卵巢组织TGF-β1、PI3K、AKT1蛋白表达明显降低(P<0.01);与模型组比较，各给药组小鼠卵巢内成熟卵泡数量均明显增多，闭锁卵泡数量相对减少，滋阴益气活血方中、高剂量组和阳性对照组小鼠卵巢组织TGF-β1、PI3K、AKT1蛋白表达明显升高(P<0.01)。结论 滋阴益气活血方可改善模型小鼠卵巢功能，其机制可能与升高TGF-β1、PI3K、AKT1蛋白的表达相关。

    关键词：滋阴益气活血法;卵巢早衰;卵巢组织;转化生长因子-β1;PI3K;AKT1;小鼠

    中图分类号：R285.5 文献标识码：A 文章编号：1005-5304(2020)01-0033-06

    DOI：10.3969/j.issn.1005-5304.201906253

Effects of Ziyin Yiqi Huoxue Prescription on Expressions of TGF-β1 and PI3K Related Proteins in Premature Ovarian Failure Mice

HUANG Shanshan， ZHANG Yunyu， LIU Huiping， ZHANG Chujie，CHEN Sha， YANG Kailin， XIAO Xiaoqin

Hunan University of Chinese Medicine， Changsha 410208， China

    Abstract： Objective To observe the effects of Ziyin Yiqi Huoxue Prescription on the expressions of transforming growth factor-β1 (TGF-β1)， PI3K and AKT1 proteins in immune premature ovarian failure mice; To explore its mechanism of action to improve ovarian function. Methods With 10 mice as blank group， fifty Balb/C female mice were subcutaneously injected with zona pellucida 3 as antigen to establish an animal model of immune premature ovarian failure. The model mice were randomly divided into model group， positive control group， Ziyin Yiqi Huoxue Prescription low-， medium-， and high-dosage groups. Administere groups were given the corresponding medicine. The medicine was administered continuously for 30 days. Pathological morphology of mouse ovary tissue was detected by HE staining. The expressions of TGF-β1， PI3K and AKT1 were detected by immunohistochemistry. Results Compared with the blank group， the expressions of TGF-β1， PI3K and AKT1 protein in ovarian tissue of model group decreased significantly (P<0.01); Compared with the model group， the number of mature follicles in ovaries in each administration groups increased significantly， and the number of atresia follicles decreased relatively. The expressions of TGF-β1， PI3K and AKT1 protein in Ziyin Yiqi Huoxue Prescription medium- and high-dosage groups and the positive control

group increased significantly (P<0.01). Conclusion Ziyin Yiqi Huoxue Prescription can improve the ovarian function of model mice， and its mechanism may be related to the increase of expressions of TGF-β1， PI3K， AKT1 protein., 百拇医药(黄姗姗 张韫玉 刘慧萍 张楚洁 陈沙 杨凯麟 肖小芹)

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