姜黄素对大鼠肝纤维化防治作用(1)
摘 要 目的：探讨姜黄素对大鼠肝纤维化的治疗作用，为临床治疗提供参考。方法：通过不同剂量姜黄素灌胃并测定血清ROS和内毒素水平、肝组织MDA含量、肝组织NF-kB活性和表达、TGF-b1、PDGF-BB、CTGF水平及TIMP-1的mRNA水平研究姜黄素对四氯化碳致大鼠实验性肝纤维化模型的作用。结果：ROS、内毒素、MDA、NF-kB、TGF-b1、PDGF-BB、CTGF、TIMP-1mRNA水平在模型组大鼠中均明显升高，与正常组比较，差异具有统计学意义(P<0.05)；给予姜黄素干预后，它们均有不同程度下降，并与剂量有一定的关系，与模型组比较，差异具有统计学意义(P<0.05)；高剂量姜黄素组各因子水平与低剂量姜黄素组比较，差异具有统计学意义(P<0.05)。结论：姜黄素对上述诸因子均有不同程度的抑制作用，具有保护肝功能、改善肝脏病理学变化等功效。
关键词 姜黄素 肝纤维化 大鼠模型
中图分类号：R575.5； R285.5 文献标识码：B 文章编号：1006-1533(2015)03-0072-04
, 百拇医药
ABSTRACT Objective： To explore the effect of curcumin on the prevention of hepatic fibrogenesis in rats so as to provide a reference for the clinical treatment. Methods： The effect of curcumin on the experimental hepatic fibrogenesis model of rats induced by carbon tetrachloride was studied by the intragastric administration of different doses of curcumin and the determination of serum ROS and endotoxin levels， the content of MDA and the activity and expression of NF-kB in liver tissue， the TGF-b1， PDGF-BB， CTGF levels and the TIMP-1 level of mRNA. Results： The levels of those factors mentioned above were significantly elevated in the model groups of rats and the differences had statistical significance compared with those in the normal group (P<0.05) and they were decreased to different extent after curcumin intervention and had some correlation with the dose of administration， and the differences had statistical significance (P<0.05) compared with those in the model groups. The differences also had statistical significance (P<0.05) when the levels in the group with higher dose of curcumin were compared with those in the group with lower dose of curcumin. Conclusion： Curcumin has different degree of inhibitory effects on the factors mentioned above， can protect the liver from fibrogenesis and improve liver pathological changes and so on.
, 百拇医药
KEY WORDS curcumin； hepatic fibrogenesis； rat model
肝纤维化是由多种原因所引起的慢性肝损害所致病理改变，是慢性肝病发展到肝硬化的必经阶段，主要表现为肝内细胞外间质成分过度异常地沉积，影响肝脏的功能[1]。姜黄素是一种酚类化合物，近年来研究发现其具有抗肝纤维化作用。但是，目前国内外学者关于姜黄素抗肝纤维化作用的研究多限在肝星状细胞水平观察姜黄素对一些细胞因子、核因子等的影响，在动物实验水平观察姜黄素防治肝纤维化作用及其机制的研究较少见。本文采用四氯化碳中毒诱导的大鼠肝纤维化模型，分析姜黄素的抗肝纤维化作用及其可能机制，现报告如下。
材料和方法
材料
雄性清洁级SD大鼠60只，体重120～160 g，由原第一军医大学动物研究所提供。
, 百拇医药
方法
分组造模
60只大鼠正常饲养1周后，随机分为A(正常组)、B(模型组)、C(高剂量姜黄素组)、D(中剂量姜黄素组)、E(低剂量姜黄素组)、F(阳性组)共6组，每组各10只。其中A组不予任何处理，其他5组大鼠给予恒量四氯化碳油溶液(天津市北宏试剂厂分析纯)0.175 ml(四氯化碳与食用花生油以1∶6的比例配制)腹腔注射造模，3次/周(周一、周三、周五各1次)，共8周。C、D、E组建模同时按每只大鼠100 g体重分别给予20、10、5 mg姜黄素灌胃(上海三爱思试剂有限公司分析纯)，3次/周，共8周；F组建模同时按每只大鼠100 g给予秋水仙碱(昆明制药集团股份有限公司)0.01 mg灌胃，1次/d，5次/周(周一、周三、周四、周五、周日各1次)，共8周。, 百拇医药(吴雄健 朱海燕)
关键词 姜黄素 肝纤维化 大鼠模型
中图分类号：R575.5； R285.5 文献标识码：B 文章编号：1006-1533(2015)03-0072-04
, 百拇医药
ABSTRACT Objective： To explore the effect of curcumin on the prevention of hepatic fibrogenesis in rats so as to provide a reference for the clinical treatment. Methods： The effect of curcumin on the experimental hepatic fibrogenesis model of rats induced by carbon tetrachloride was studied by the intragastric administration of different doses of curcumin and the determination of serum ROS and endotoxin levels， the content of MDA and the activity and expression of NF-kB in liver tissue， the TGF-b1， PDGF-BB， CTGF levels and the TIMP-1 level of mRNA. Results： The levels of those factors mentioned above were significantly elevated in the model groups of rats and the differences had statistical significance compared with those in the normal group (P<0.05) and they were decreased to different extent after curcumin intervention and had some correlation with the dose of administration， and the differences had statistical significance (P<0.05) compared with those in the model groups. The differences also had statistical significance (P<0.05) when the levels in the group with higher dose of curcumin were compared with those in the group with lower dose of curcumin. Conclusion： Curcumin has different degree of inhibitory effects on the factors mentioned above， can protect the liver from fibrogenesis and improve liver pathological changes and so on.
, 百拇医药
KEY WORDS curcumin； hepatic fibrogenesis； rat model
肝纤维化是由多种原因所引起的慢性肝损害所致病理改变，是慢性肝病发展到肝硬化的必经阶段，主要表现为肝内细胞外间质成分过度异常地沉积，影响肝脏的功能[1]。姜黄素是一种酚类化合物，近年来研究发现其具有抗肝纤维化作用。但是，目前国内外学者关于姜黄素抗肝纤维化作用的研究多限在肝星状细胞水平观察姜黄素对一些细胞因子、核因子等的影响，在动物实验水平观察姜黄素防治肝纤维化作用及其机制的研究较少见。本文采用四氯化碳中毒诱导的大鼠肝纤维化模型，分析姜黄素的抗肝纤维化作用及其可能机制，现报告如下。
材料和方法
材料
雄性清洁级SD大鼠60只，体重120～160 g，由原第一军医大学动物研究所提供。
, 百拇医药
方法
分组造模
60只大鼠正常饲养1周后，随机分为A(正常组)、B(模型组)、C(高剂量姜黄素组)、D(中剂量姜黄素组)、E(低剂量姜黄素组)、F(阳性组)共6组，每组各10只。其中A组不予任何处理，其他5组大鼠给予恒量四氯化碳油溶液(天津市北宏试剂厂分析纯)0.175 ml(四氯化碳与食用花生油以1∶6的比例配制)腹腔注射造模，3次/周(周一、周三、周五各1次)，共8周。C、D、E组建模同时按每只大鼠100 g体重分别给予20、10、5 mg姜黄素灌胃(上海三爱思试剂有限公司分析纯)，3次/周，共8周；F组建模同时按每只大鼠100 g给予秋水仙碱(昆明制药集团股份有限公司)0.01 mg灌胃，1次/d，5次/周(周一、周三、周四、周五、周日各1次)，共8周。, 百拇医药(吴雄健 朱海燕)