喻莹 陈建伟 陈志禹

    摘 要 川贝母是中国最常用的化痰止咳贵重药草，贝母属植物种类繁多，各种属来源复杂，川贝母与其混伪品之间鉴别困难，近年来分子生物技术在川贝母鉴定中开始了大规模的应用研究，如PCR-RFLP、PCR-RAPD、SSR、PCRISSR、qPCR、DNA条形码、SNP、PCR-AFLP等技术的应用，并且展现出较好的应用前景。

    关键词 分子生物技术 川贝母 鉴别

    中图分类号：R282.5 文献标志码：A 文章编号：1006-1533(2021)05-0073-04

    *基金项目：宁波市自然科学基金项目“realtime PCR用于检定川贝母掺伪情况的定量研究”(2018A610429)

    Prospect of the application of molecular biotechnology in identification of Fritillaria cirrhosa*

    YU Ying1**， CHEN Jianwei2***， CHEN Zhiyu2

    (1. Zhejiang University of Traditional Chinese Medicine， Hangzhou 310053， China； 2. Ningbo Institute for Drug Inspection， Zhejiang Ningbo 315000， China)

    ABSTRACT Bulbus Fritillariae cirrhosae is phlegm cough precious herbs that is the most commonly used in China. There are many different kinds of plants of the genus Fritillaria and the sources of various genus are complex. The identification between Bulbus Fritillariae cirrhosae and the adulterants are difficult. Molecular biotechnology has begun a large-scale study in identification of Bulbus Fritillariae cirrhosae in recent years ......