李荣胜 张玉杰 林超颖 潘一峰

    摘 要 目的：建立松花粉中柚皮素HPLC法测定方法，提高松花粉质量标准。方法：采用高效液相色谱法(HPLC)测定柚皮素含量，以甲醇-0.05%磷酸溶液(45∶55)为流动相，检测波长为288 nm测定柚皮素的含量。结果：柚皮素质量浓度在10.0～216.0 μg/ml范围内线性关系良好(r=1.000 0)，平均回收率99.26% ，RSD为1.04%(n=9)。运用该方法测得12批次不同产地的松花粉中柚皮素含量在0.13%～0.27%之间。结论：建立的HPLC分析方法操作简便、快速、专属性强、重复性好，可作为该松花粉的质量控制方法。

    关键词 松花粉 柚皮素 高效液相色谱

    中图分类号：R282.71； O657.72 文献标志码：A 文章编号：1006-1533(2021)09-0064-03

    *基金项目：国家科技重大专项(民口)课题重大新药创制专项“扶正化瘀片抗肝纤维化的CMC及美国Ⅲ期临床试验”(2018ZX09737010)

    Study on an HPLC method for the determination of naringenin content in pini pollen*

    LI Rongsheng**， ZHANG Yujie， LIN Chaoying， PAN Yifeng***

    (Shanghai Huanghai Pharmaceutical Co.， LTD.， Shanghai 200942， China)

    ABSTRACT Objective： To establish an HPLC method for the determination of pini pollen naringenin to improve its quality standard. Methods： The content of naringenin was determined by high performance liquid chromatography (HPLC) at 288 nm using methanol-0.05% phosphoric acid solution (45：55) as mobile phase. Results： The standard curve of naringenin showed good linearity in the range of 10.0-216.0 μg/ml (r=1.000 0). The average recovery was 99.26% with RSD 1.04% (n=9). The contents of naringenin in 12 batches of pini pollen from different origins were in the range of 0.13%-0.27%. Conclusion： The established HPLC method is simple ......