盐酸法舒地尔对EAE小鼠脑中ROCK-II表达的影响(1)
http://www.100md.com
2010年5月1日
 |
| 第1页 |
参见附件(1427KB，2页)。
【摘要】目的:探讨盐酸法舒地尔(Fasudil hydrochloride)对实验性自身免疫性脑脊髓炎(EAE)小鼠脑中Rho激酶-Ⅱ(ROCK-Ⅱ)表达的影响。方法:雌性C57BL/6小鼠,分为EAE组、治疗组和佐剂组。用MOG35-55多肽诱导EAE模型。治疗组于d6p.i.开始腹腔注射盐酸法舒地尔液,连续14天。观察和比较各组临床表现,于d18-20p.i.取脊髓和脑,行HE染色、Western blot法检测各组ROCK-II的表达。结果:盐酸法舒地尔能够显著改善EAE的症状,减轻中枢神经系统(CNS)炎性浸润等病理变化。各组小鼠脑中均有ROCK-Ⅱ的表达,EAE组ROCK-Ⅱ表达量较佐剂组要高(P<0.05),治疗组与EAE组相比显著减低(P<0.05)。结论:盐酸法舒地尔可以有效抑制小鼠EAE,这种抑制可能与其抑制ROCK-II的活化有关。
【关键词】实验性自身免疫性脑脊髓炎;多发性硬化;盐酸法舒地尔;ROCK-II
doi:10.3969/j.issn.1006-1959.2010.05.016 文章编号:1006-1959(2010)-05-1043-02
Effect of Fasudil hydrochloride on ROCK-Ⅱexpression in brains of experimental autoimmune encephalomyelitis (EAE) mice DUAN Hui-jie1,2,LIU Jin-quan1,2,GUO Min-fang1,YU Jie-zhong1,LIANG Li-yun1,MA Cun-gen1. 1.Institute of rain Science,Shanxi Datong University,Datong 037009 China;2.Department of Neurology,the First Clinical Medical College,Shanxi Medical University,Taiyuan 030001,China
【Abstract】Objective:To investigate the effect of Fasudil hydrochloride on expression of ROCK-Ⅱin brains of experimental autoimmune encephalomyelitis(EAE) mice.Methods:female C57BL/6 mice were divided by average body weight into EAE group,Fasudil hydrochloride treated group and Adjuvant group.Mice were immunized subcutaneously with MOG35-55.Fasudil hydrochloride was intraperitoneally injected in treated group from day 6 post-immunization(p.i),and followed by 14 days.The clinical manifestations of each group were observed and compared.Mice were sacrificed on days 18-20 p.i.The spinal cords were dissected for HE.The brains were dissected for Western blot to detect the expression of ROCK-Ⅱ.Data were analyzed with SPSS13.0 software.Results:Fasudil hydrochloride may significant ameliorate symptoms of EAE mice,lessen inflammatory cell infiltration.The expression of ROCK-Ⅱin brain of EAE mice increased obviously compared with that of adjuvant group(P<0.05).After intervention of Fasudil hydrochloride,the expression of ROCK-Ⅱ was statistically decreased compared with that of EAE group(P<0 ......
您现在查看是摘要介绍页，详见PDF附件(1427KB，2页)。